TY - JOUR
T1 - Dyes as fungal inhibitors: effect on colony enumeration
AU - Bragulat, M. R.
AU - Abarca, M. L.
AU - Castellá, G.
AU - Cabañes, F. J.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - The effects of three dyes on the colony enumeration of nine fungal strains (including members of the Deuteromycetes and Zygomycetes) in pure and mixed cultures were investigated. Using malt extract agar as basal and control medium, the following dyes and concentrations were assayed: auramine (25 ppm), gentian violet (5 ppm) and malachite green (1 ppm). The chemicals commonly used in commercial media dichloran (2 ppm) and rose bengal (50 ppm) were included in the study as reference mould‐spreading inhibitors. Higher counts were usually obtained in the media containing dichloran, rose bengal or auramine, including the control medium in the absence of chemical when the mixed‐conidium inocula did not include a spreading mould. Nevertheless in most cases no significant differences were observed between them. Malachite green (1 ppm) performed mainly as a strong inhibitor of spreading moulds, only allowing adequate colony development and recoveries of both Fusarium and Aspergillus strains tested. Copyright © 1995, Wiley Blackwell. All rights reserved
AB - The effects of three dyes on the colony enumeration of nine fungal strains (including members of the Deuteromycetes and Zygomycetes) in pure and mixed cultures were investigated. Using malt extract agar as basal and control medium, the following dyes and concentrations were assayed: auramine (25 ppm), gentian violet (5 ppm) and malachite green (1 ppm). The chemicals commonly used in commercial media dichloran (2 ppm) and rose bengal (50 ppm) were included in the study as reference mould‐spreading inhibitors. Higher counts were usually obtained in the media containing dichloran, rose bengal or auramine, including the control medium in the absence of chemical when the mixed‐conidium inocula did not include a spreading mould. Nevertheless in most cases no significant differences were observed between them. Malachite green (1 ppm) performed mainly as a strong inhibitor of spreading moulds, only allowing adequate colony development and recoveries of both Fusarium and Aspergillus strains tested. Copyright © 1995, Wiley Blackwell. All rights reserved
U2 - 10.1111/j.1365-2672.1995.tb03179.x
DO - 10.1111/j.1365-2672.1995.tb03179.x
M3 - Article
SN - 0021-8847
VL - 79
SP - 578
EP - 582
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
IS - 5
ER -