Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

Semih Sevim, Sevil Ozer, Luying Feng, Joel Wurzel, Arielle Fakhraee, Naveen Shamsudhin, Bumjin Jang, Carlos Alcantara, Olgaç Ergeneman, Eva Pellicer, Jordi Sort, Tessa Lühmann, Salvador Pané, Bradley J. Nelson, Hamdi Torun

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3 Citations (Scopus)

Abstract

© 2018 The Royal Society of Chemistry. We report a novel atomic force microscopy (AFM) technique with dual actuation capabilities using both piezo and magnetic bead actuation for advanced single-molecule force spectroscopy experiments. The experiments are performed by manipulating magnetic microbeads using an electromagnet against a stationary cantilever. Magnetic actuation has been demonstrated before to actuate cantilevers, but here we keep the cantilever stationary and accomplish actuation via free-manipulated microstructures. The developed method benefits from significant reduction of drift, since the experiments are performed without a substrate contact and the measured force is inherently differential. In addition, shrinking the size of the actuator can minimize hydrodynamic forces affecting the cantilever. The new method reported herein allows for the application of constant force to perform force-clamp experiments without any active feedback, profiled for a deeper understanding of biomolecular interactions.
Original languageEnglish
Pages (from-to)488-495
JournalNanoscale Horizons
Volume1
DOIs
Publication statusPublished - 1 Nov 2016

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    Sevim, S., Ozer, S., Feng, L., Wurzel, J., Fakhraee, A., Shamsudhin, N., Jang, B., Alcantara, C., Ergeneman, O., Pellicer, E., Sort, J., Lühmann, T., Pané, S., Nelson, B. J., & Torun, H. (2016). Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements. Nanoscale Horizons, 1, 488-495. https://doi.org/10.1039/c6nh00134c