Dual chronoamperometric detection of enzymatic biomarkers using magnetic beads and a low-cost flow cell

Javier Moral-Vico, Jaume Barallat, Llibertat Abad, Rosa Olivé-Monllau, Francesc Xavier Muñoz-Pascual, Amparo Galán Ortega, F. Javier del Campo, Eva Baldrich

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)


© 2015 Elsevier B.V. In this work we report on the production of a low cost microfluidic device for the multiplexed electrochemical detection of magneto bioassays. As a proof of concept, the device has been used to detect myeloperoxidase (MPO), a cardiovascular biomarker. With this purpose, two bioassays have been optimized in parallel onto magnetic beads (MBs) for the simultaneous detection of MPO endogenous peroxidase activity and quantification of total MPO. Since the two bioassays produced signals of different magnitude for each concentration of MPO tested, two detection strategies have been compared, which entailed registering steady state currents (Iss) under substrate flow, and measuring the peak currents (Ip) produced in a stopped flow approach. As it will be shown, appropriate tuning of the detection and flow conditions can provide extremely sensitive detection, but also allow simultaneous detection of assays or parameters that would produce signals of different orders of magnitude when measured by a single detection strategy. In order to demonstrate the feasibility of the detection strategy reported, a dual MPO mass and activity assay has been finally applied to the study of 10 real plasma samples, allowing patient classification according to the risk of suffering a cardiovascular event.
Original languageEnglish
Pages (from-to)328-336
JournalBiosensors and Bioelectronics
Publication statusPublished - 5 Jul 2015


  • Cardiovascular disease
  • Electrochemical detection
  • Enzyme biomarker
  • Low-Cost flow cell
  • Magneto-Immunosensor
  • Myeloperoxidase (MPO)


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