TY - JOUR
T1 - Dual chronoamperometric detection of enzymatic biomarkers using magnetic beads and a low-cost flow cell
AU - Moral-Vico, Javier
AU - Barallat, Jaume
AU - Abad, Llibertat
AU - Olivé-Monllau, Rosa
AU - Muñoz-Pascual, Francesc Xavier
AU - Galán Ortega, Amparo
AU - del Campo, F. Javier
AU - Baldrich, Eva
PY - 2015/7/5
Y1 - 2015/7/5
N2 - © 2015 Elsevier B.V. In this work we report on the production of a low cost microfluidic device for the multiplexed electrochemical detection of magneto bioassays. As a proof of concept, the device has been used to detect myeloperoxidase (MPO), a cardiovascular biomarker. With this purpose, two bioassays have been optimized in parallel onto magnetic beads (MBs) for the simultaneous detection of MPO endogenous peroxidase activity and quantification of total MPO. Since the two bioassays produced signals of different magnitude for each concentration of MPO tested, two detection strategies have been compared, which entailed registering steady state currents (Iss) under substrate flow, and measuring the peak currents (Ip) produced in a stopped flow approach. As it will be shown, appropriate tuning of the detection and flow conditions can provide extremely sensitive detection, but also allow simultaneous detection of assays or parameters that would produce signals of different orders of magnitude when measured by a single detection strategy. In order to demonstrate the feasibility of the detection strategy reported, a dual MPO mass and activity assay has been finally applied to the study of 10 real plasma samples, allowing patient classification according to the risk of suffering a cardiovascular event.
AB - © 2015 Elsevier B.V. In this work we report on the production of a low cost microfluidic device for the multiplexed electrochemical detection of magneto bioassays. As a proof of concept, the device has been used to detect myeloperoxidase (MPO), a cardiovascular biomarker. With this purpose, two bioassays have been optimized in parallel onto magnetic beads (MBs) for the simultaneous detection of MPO endogenous peroxidase activity and quantification of total MPO. Since the two bioassays produced signals of different magnitude for each concentration of MPO tested, two detection strategies have been compared, which entailed registering steady state currents (Iss) under substrate flow, and measuring the peak currents (Ip) produced in a stopped flow approach. As it will be shown, appropriate tuning of the detection and flow conditions can provide extremely sensitive detection, but also allow simultaneous detection of assays or parameters that would produce signals of different orders of magnitude when measured by a single detection strategy. In order to demonstrate the feasibility of the detection strategy reported, a dual MPO mass and activity assay has been finally applied to the study of 10 real plasma samples, allowing patient classification according to the risk of suffering a cardiovascular event.
KW - Cardiovascular disease
KW - Electrochemical detection
KW - Enzyme biomarker
KW - Low-Cost flow cell
KW - Magneto-Immunosensor
KW - Myeloperoxidase (MPO)
U2 - 10.1016/j.bios.2015.02.042
DO - 10.1016/j.bios.2015.02.042
M3 - Article
VL - 69
SP - 328
EP - 336
ER -