Dual carbon - chlorine isotope fractionation during dichloroelimination of 1,1,2-trichloroethane by an enrichment culture containing Dehalogenimonas sp

Mònica Rosell, Jordi Palau, Siti Hatijah Mortan, Gloria Caminal, Albert Soler, Orfan Shouakar-Stash, Ernest Marco-Urrea

Research output: Contribution to journalArticleResearch

5 Citations (Scopus)

Abstract

© 2018 Elsevier B.V. Chlorinated ethanes are frequent groundwater contaminants but compound specific isotope analysis (CSIA) has been scarcely applied to investigate their degradation pathways. In this study, dual carbon and chlorine isotope fractionation was used to investigate for the first time the anoxic biodegradation of 1,1,2-trichloroethane (1,1,2-TCA) using a Dehalogenimonas-containing culture. The isotopic fractionation values obtained for the biodegradation of 1,1,2-TCA were ɛC = −6.9 ± 0.4‰ and ɛCl = −2.7 ± 0.3‰. The detection of vinyl chloride (VC) as unique byproduct and a closed carbon isotopic mass balance corroborated that dichloroelimination was the degradation pathway used by this strain. Combining the values of δ13C and δ37Cl resulted in a dual element C-Cl isotope slope of Λ = 2.5 ± 0.2‰. Investigation of the apparent kinetic isotope effects (AKIEs) expected for cleavage of a C–Cl bond showed an important masking of the intrinsic isotope fractionation. Theoretical calculation of Λ suggested that dichloroelimination of 1,1,2-TCA was taking place via simultaneous cleavage of two C–Cl bonds (concerted reaction mechanism). The isotope data obtained in this study can be useful to monitor natural attenuation of 1,1,2-TCA via dichloroelimination and provide insights into the source and fate of VC in contaminated groundwaters.
Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalScience of the Total Environment
Volume648
DOIs
Publication statusPublished - 15 Jan 2019

Keywords

  • 1,1,2-Trichloroethane
  • ABIOTIC DEGRADATION
  • ALIPHATIC-COMPOUNDS
  • BIODEGRADATION
  • Biodegradation, Environmental
  • Carbon Isotopes/analysis
  • Chemical Fractionation
  • Chlorine/analysis
  • Chloroflexi/metabolism
  • DEGRADATION PATHWAYS
  • DEHALOCOCCOIDES-MCCARTYI STRAINS
  • Dehalogenimonas
  • Dichloroelimination
  • Dual isotope fractionation
  • ETHANES
  • Isotopes/analysis
  • Organohalide-respiring bacteria
  • REDUCTIVE DECHLORINATION
  • TRANSFORMATION PATHWAYS
  • TRICHLOROETHENE TCE
  • Trichloroethanes/metabolism
  • VINYL-CHLORIDE
  • Waste Disposal, Fluid/methods
  • Water Pollutants, Chemical/metabolism

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