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Abstract
Grafting biomolecules on nanostructures' surfaces is an increasingly used strategy to target pathogenic cells, with both diagnostic and therapeutic applications. However, nanomaterials monofunctionalized by conjugating a single type of ligand find limited uses in pathologies/therapies that require two or more targets/receptors to be targeted and/or activated with a single molecular entity simultaneously. Therefore, multivalent nanomaterials for dual- or multitargeting are attracting significant interest. This study provides a proof of concept of such nanostructures. We have recently developed a modular methodology that allows obtaining amyloid-based materials decorated with active globular domains. Here, this approach is exploited to generate functional amyloid fibrils displaying antibody capture moieties. A high antibody binding affinity and capacity for the resulting nanofibrils, whose size can be manipulated to obtain homogeneous nanorods with high biocompatibility, are demonstrated. These nanorods are then used for specific antibody-mediated targeting of different cell types. Simultaneous conjugation of these nanorods with different antibodies allows obtaining a mimic of a bispecific antibody that redirects T lymphocytes to tumoral cells, holding high potential for immunotherapy. Overall, the work illustrates a modular and straightforward strategy to obtain preparative quantities of multivalent antibody-functionalized nanomaterials with multitargeting properties without the need for covalent modification.
Original language | English |
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Pages (from-to) | 14875-14884 |
Number of pages | 10 |
Journal | ACS Applied Materials & Interfaces |
Volume | 13 |
Issue number | 13 |
DOIs | |
Publication status | Published - 7 Apr 2021 |
Keywords
- amyloid
- antibody
- dual- or multitargeting
- multivalency
- nanomaterials
- nanorods
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Dive into the research topics of 'Dual Antibody-Conjugated Amyloid Nanorods to Promote Selective Cell-Cell Interactions'. Together they form a unique fingerprint.Projects
- 2 Finished
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PROTEIN AGGREGATION: BIOMEDICAL AND BIOTECHNOLOGICAL APPLICATIONS
Ventura Zamora, S., Batlle Carreras, C., Baño Polo, M., Diaz Caballero, M., Garcia de Carvalho Pinheiro, F., Iglesias Mas, J. V., Navarro Cantero, S., Peña Diaz, S., Pujols Pujol, J., Fernandez Gallegos, M. R., Pallares Goitiz, I., Gil-Garcia, M., SANTOS SUAREZ, J., Wang, W., Garcia Pardo, J. & Behbahanipour ., M.
1/06/20 → 31/05/23
Project: Research Projects and Other Grants
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Agregación de proteínas: estrategias terapéuticas, diagnóstico y aplicaciones
Ventura Zamora, S., Bartolomé Piñol, S., Fernandez Gallegos, M. R., Pallares Goitiz, I., Navarro Cantero, S. & Peña Diaz, S.
30/12/16 → 31/12/20
Project: Research Projects and Other Grants