Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains

Elena Cámara, Joan Albiol, Pau Ferrer

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

© 2015 Wiley Periodicals, Inc. Pichia (syn. Komagataella) pastoris is a widely used yeast platform for heterologous protein production. Expression cassettes are usually stably integrated into the genome of this host via homologous recombination. Although increasing gene dosage is a powerful strategy to improve recombinant protein production, an excess in the number of gene copies often leads to decreased product yields and increased metabolic burden, particularly for secreted proteins. We have constructed a series of strains harboring different copy numbers of a Rhizopus oryzae lipase gene (ROL), aiming to find the optimum gene dosage for secreted Rol production. In order to accurately determine ROL gene dosage, we implemented a novel protocol based on droplet digital PCR (ddPCR), and cross validated it with conventional real-time PCR. Gene copy number determination based on ddPCR allowed for an accurate ranking of transformants according to their ROL gene dosage. Results indicated that ddPCR was particularly superior at lower gene dosages (one to five copies) over quantitative real-time PCR (qPCR). This facilitated the determination of the optimal ROL gene dosage as low as two copies. The ranking of ROL gene dosage versus Rol yield was consistent at both small scale and bioreactor chemostat cultures, thereby easing clone characterization in terms of gene dosage dependent physiological effects, which could be discriminated even among strains differing by only one ROL copy. A selected two-copy strain showed twofold increase in Rol specific production in a chemostat culture over the single copy strain. Conversely, strains harboring more than two copies of the ROL gene showed decreased product and biomass yields, as well as altered substrate consumption specific rates, compared to the reference (one-copy) strain.
Original languageEnglish
Pages (from-to)1542-1551
JournalBiotechnology and Bioengineering
Volume113
Issue number7
DOIs
Publication statusPublished - 1 Jul 2016

Keywords

  • DdPCR
  • Gene dosage
  • Pichia pastoris
  • QPCR
  • Recombinant protein
  • Rhizopus oryzae lipase

Fingerprint Dive into the research topics of 'Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains'. Together they form a unique fingerprint.

Cite this