Purpose: To assess the DNA damage induced by MBRT and BB radiations on glioma cells. Methods: The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death. Results: A higher γH2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G 1 and G2 phases. Conclusions: A pronounced G2 phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G1 phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy. © 2013 Federación de Sociedades Españolas de Oncología (FESEO).
|Journal||Clinical and Translational Oncology|
|Publication status||Published - 1 Jan 2014|
- Double-strand breaks
- Nuclear damage
- Synchrotron radiation
- Threshold dose