Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy

S. Gil, Y. Prezado, M. Sabés

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Purpose: To assess the DNA damage induced by MBRT and BB radiations on glioma cells. Methods: The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death. Results: A higher γH2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G 1 and G2 phases. Conclusions: A pronounced G2 phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G1 phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy. © 2013 Federación de Sociedades Españolas de Oncología (FESEO).
Original languageEnglish
Pages (from-to)696-701
JournalClinical and Translational Oncology
Volume16
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Double-strand breaks
  • Gliomas
  • Minibeams
  • Nuclear damage
  • Synchrotron radiation
  • Threshold dose

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