DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis

F. Papa; M. Luquin; H. L. David

doi:10.1016/0923-2508(92)90024-I

DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis

F. Papa, M. Luquin, H. L. David

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb 1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species. © 1992.

Original language	English
Pages (from-to)	327-331
Journal	Research in Microbiology
Volume	143
Issue number	3
DOIs	https://doi.org/10.1016/0923-2508(92)90024-I
Publication status	Published - 1 Jan 1992

Keywords

DAT
Détection
DOT-ELISA
Glycolipid
Mycobacterium tuberculosis
PGL-Tb1

Access to Document

10.1016/0923-2508(92)90024-I

Fingerprint Dive into the research topics of 'DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis'. Together they form a unique fingerprint.

Cite this

@article{021e5eb143c84d97992f957c5b6cbe8a,

title = "DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis",

abstract = "A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb 1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species. {\textcopyright} 1992.",

keywords = "DAT, D{\'e}tection, DOT-ELISA, Glycolipid, Mycobacterium tuberculosis, PGL-Tb1",

author = "F. Papa and M. Luquin and David, {H. L.}",

year = "1992",

month = jan,

day = "1",

doi = "10.1016/0923-2508(92)90024-I",

language = "English",

volume = "143",

pages = "327--331",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson SAS",

number = "3",

}

TY - JOUR

T1 - DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis

AU - Papa, F.

AU - Luquin, M.

AU - David, H. L.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb 1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species. © 1992.

AB - A DOT-ELISA method for detection of 2,3-diacyl-trehalose (DAT, previously referred to as SL-IV antigen) and triglycosyl phenol phthiocerol di-mycocerosate (PGL-Tb 1) antigens from Mycobacterium tuberculosis is described. The method enabled the detection of both antigens in 14 clinical isolates of M. tuberculosis from different geographic origins; the presence of the glycolipids was confirmed by chemical analysis. It was therefore concluded that the synthesis of both of these compounds is characteristic of the species. © 1992.

KW - DAT

KW - Détection

KW - DOT-ELISA

KW - Glycolipid

KW - Mycobacterium tuberculosis

KW - PGL-Tb1

U2 - 10.1016/0923-2508(92)90024-I

DO - 10.1016/0923-2508(92)90024-I

M3 - Article

VL - 143

SP - 327

EP - 331

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 3

ER -