Does Ca2+/Mg2+-free medium have an effect on the survival of the preimplantation mouse embryo after biopsy?

J. Santaló, M. Grossmann, J. Egozcue

    Research output: Contribution to journalReview articleResearchpeer-review

    18 Citations (Scopus)

    Abstract

    The way in which the different technical aspects of clinical preimplantation genetic diagnosis affect the survival of the biopsied embryo is not well understood. One of these aspects is the influence of Ca2+/Mg2+-free medium on the developmental capacity of the biopsied embryo and the biopsied cell itself. Therefore, we used an experimental design involving 4-cell mouse embryos to evaluate the effect of performing the biopsy procedure under such conditions. Our results indicate that the use of Ca2+/Mg2+-free medium has no detrimental effect (at least in mouse embryos) on the viability of the biopsied embryos and their biopsied cells when used during relatively short times of exposure.
    Original languageEnglish
    Pages (from-to)257-261
    JournalHuman Reproduction Update
    Volume2
    Issue number3
    DOIs
    Publication statusPublished - 1 May 1996

    Keywords

    • Ca /Mg -free medium 2+ 2+
    • Preimplantation diagnosis

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