DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes

Marta I. Sierra; Laura Rubio; Gustavo F. Bayón; Isabel Cobo; Pablo Menendez; Paula Morales; Cristina Mangas; Rocio G. Urdinguio; Virginia Lopez; Adolfo Valdes; Gerard Vales; Ricard Marcos; Ramon Torrecillas; Agustin F. Fernández; Mario F. Fraga

doi:10.1080/17435390.2017.1371350

DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes

Marta I. Sierra, Laura Rubio, Gustavo F. Bayón, Isabel Cobo, Pablo Menendez, Paula Morales, Cristina Mangas, Rocio G. Urdinguio, Virginia Lopez, Adolfo Valdes, Gerard Vales, Ricard Marcos, Ramon Torrecillas, Agustin F. Fernández, Mario F. Fraga

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33 Citations (Scopus)

Abstract

© 2017 Informa UK Limited, trading as Taylor & Francis Group. Humans are increasingly exposed to nanoparticles and, although many of their physiological effects have been described, the molecular mechanisms underlying them are still largely unknown. The present study aimed to determine the possible role of certain epigenetic mechanisms in the cellular response of human lung epithelial cells that are triggered by long-term exposure to titanium dioxide nanoparticles (TiO2NPs) and multi-walled carbon nanotubes (MWCNTs). The results showed that exposure to TiO2NPs had only minor effects on genome-wide DNA methylation. However, we identified 755 CpG sites showing consistent DNA hypomethylation in cells exposed to MWCNTs. These sites were mainly located at low density CpG regions and enhancers, and very frequently on the X chromosome. Our results thus suggest that long-term MWCNT exposure may have important effects on the epigenome.

Original language	English
Pages (from-to)	857-870
Journal	Nanotoxicology
Volume	11
Issue number	7
DOIs	https://doi.org/10.1080/17435390.2017.1371350
Publication status	Published - 9 Aug 2017

Keywords

DNA methylation
Epigenetics
exposure
nanomaterials

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10.1080/17435390.2017.1371350

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Sierra, M. I., Rubio, L., Bayón, G. F., Cobo, I., Menendez, P., Morales, P., Mangas, C., Urdinguio, R. G., Lopez, V., Valdes, A., Vales, G., Marcos, R., Torrecillas, R., Fernández, A. F., & Fraga, M. F. (2017). DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes. Nanotoxicology, 11(7), 857-870. https://doi.org/10.1080/17435390.2017.1371350

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title = "DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes",

abstract = "{\textcopyright} 2017 Informa UK Limited, trading as Taylor & Francis Group. Humans are increasingly exposed to nanoparticles and, although many of their physiological effects have been described, the molecular mechanisms underlying them are still largely unknown. The present study aimed to determine the possible role of certain epigenetic mechanisms in the cellular response of human lung epithelial cells that are triggered by long-term exposure to titanium dioxide nanoparticles (TiO2NPs) and multi-walled carbon nanotubes (MWCNTs). The results showed that exposure to TiO2NPs had only minor effects on genome-wide DNA methylation. However, we identified 755 CpG sites showing consistent DNA hypomethylation in cells exposed to MWCNTs. These sites were mainly located at low density CpG regions and enhancers, and very frequently on the X chromosome. Our results thus suggest that long-term MWCNT exposure may have important effects on the epigenome.",

keywords = "DNA methylation, Epigenetics, exposure, nanomaterials",

author = "Sierra, {Marta I.} and Laura Rubio and Bay{\'o}n, {Gustavo F.} and Isabel Cobo and Pablo Menendez and Paula Morales and Cristina Mangas and Urdinguio, {Rocio G.} and Virginia Lopez and Adolfo Valdes and Gerard Vales and Ricard Marcos and Ramon Torrecillas and Fern{\'a}ndez, {Agustin F.} and Fraga, {Mario F.}",

year = "2017",

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doi = "10.1080/17435390.2017.1371350",

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Sierra, MI, Rubio, L, Bayón, GF, Cobo, I, Menendez, P, Morales, P, Mangas, C, Urdinguio, RG, Lopez, V, Valdes, A, Vales, G, Marcos, R, Torrecillas, R, Fernández, AF & Fraga, MF 2017, 'DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes', Nanotoxicology, vol. 11, no. 7, pp. 857-870. https://doi.org/10.1080/17435390.2017.1371350

TY - JOUR

T1 - DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes

AU - Sierra, Marta I.

AU - Rubio, Laura

AU - Bayón, Gustavo F.

AU - Cobo, Isabel

AU - Menendez, Pablo

AU - Morales, Paula

AU - Mangas, Cristina

AU - Urdinguio, Rocio G.

AU - Lopez, Virginia

AU - Valdes, Adolfo

AU - Vales, Gerard

AU - Marcos, Ricard

AU - Torrecillas, Ramon

AU - Fernández, Agustin F.

AU - Fraga, Mario F.

PY - 2017/8/9

Y1 - 2017/8/9

N2 - © 2017 Informa UK Limited, trading as Taylor & Francis Group. Humans are increasingly exposed to nanoparticles and, although many of their physiological effects have been described, the molecular mechanisms underlying them are still largely unknown. The present study aimed to determine the possible role of certain epigenetic mechanisms in the cellular response of human lung epithelial cells that are triggered by long-term exposure to titanium dioxide nanoparticles (TiO2NPs) and multi-walled carbon nanotubes (MWCNTs). The results showed that exposure to TiO2NPs had only minor effects on genome-wide DNA methylation. However, we identified 755 CpG sites showing consistent DNA hypomethylation in cells exposed to MWCNTs. These sites were mainly located at low density CpG regions and enhancers, and very frequently on the X chromosome. Our results thus suggest that long-term MWCNT exposure may have important effects on the epigenome.

AB - © 2017 Informa UK Limited, trading as Taylor & Francis Group. Humans are increasingly exposed to nanoparticles and, although many of their physiological effects have been described, the molecular mechanisms underlying them are still largely unknown. The present study aimed to determine the possible role of certain epigenetic mechanisms in the cellular response of human lung epithelial cells that are triggered by long-term exposure to titanium dioxide nanoparticles (TiO2NPs) and multi-walled carbon nanotubes (MWCNTs). The results showed that exposure to TiO2NPs had only minor effects on genome-wide DNA methylation. However, we identified 755 CpG sites showing consistent DNA hypomethylation in cells exposed to MWCNTs. These sites were mainly located at low density CpG regions and enhancers, and very frequently on the X chromosome. Our results thus suggest that long-term MWCNT exposure may have important effects on the epigenome.

KW - DNA methylation

KW - Epigenetics

KW - exposure

KW - nanomaterials

U2 - 10.1080/17435390.2017.1371350

DO - 10.1080/17435390.2017.1371350

M3 - Article

VL - 11

SP - 857

EP - 870

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

IS - 7

ER -

DNA methylation changes in human lung epithelia cells exposed to multi-walled carbon nanotubes

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