DNA hybridization detection by electrochemical impedance spectroscopy using interdigitated gold nanoelectrodes

Alessandra Bonanni, Irene Fernández-Cuesta, Xavier Borrisé, Francesc Pérez-Murano, Salvador Alegret, Manel del Valle

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

A DNA biosensor is presented that is based on gold interdigitated nanoelectrodes of 100 nm width and 250 nm pitch. A single-strand oligonucleotide acts as the capture probe. Because of its nanometer dimensions, the device shows improved sensitivity when compared to similar systems. This encouraged us to perform a direct and unlabelled detection. After incubation with the DNA target, the impedance spectrum was recorded between 1 kHz and 10 MHz to obtain the net capacitance change. The use of a biotinylated DNA signalling probe permitted the integration of an amplification stage in a sandwich format that employs streptavidin-modified gold nanoparticles. The strategy was preliminarily tested by detecting the breast cancer related BRCA1 gene, where the noncomplementary, wild and mutated forms were easily differentiated at a concentration level of 3 μM (corresponding to a 30 pmol quantity). © 2010 Springer-Verlag.
Original languageEnglish
Pages (from-to)275-281
JournalMicrochimica Acta
Volume170
Issue number3
DOIs
Publication statusPublished - 29 Apr 2010

Keywords

  • BRCA1 gene
  • Gold nanoparticle amplification
  • Impedimetric genosensor
  • Interdigitated electrodes
  • Nanoelectrodes

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