DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Stefano Bonassi; Marcello Ceppi; Peter Møller; Amaya Azqueta; Mirta Milić; Neri Monica; Gunnar Brunborg; Roger Godschalk; Gudrun Koppen; Sabine A.S. Langie; João Paulo Teixeira; Marco Bruzzone; Juliana Da Silva; Danieli Benedetti; Delia Cavallo; Cinzia Lucia Ursini; Lisa Giovannelli; Silvia Moretti; Patrizia Riso; Cristian Del Bo’; Patrizia Russo; Malgorzata Dobrzyńska; Irina A. Goroshinskaya; Ekaterina I. Surikova; Marta Staruchova; Magdalena Barančokova; Katarina Volkovova; Alena Kažimirova; Bozena Smolkova; Blanca Laffon; Vanessa Valdiglesias; Susana Pastor; Ricard Marcos; Alba Hernández; Goran Gajski; Biljana Spremo-Potparević; Lada Živković; Elisa Boutet-Robinet; Hervé Perdry; Pierre Lebailly; Carlos L. Perez; Nursen Basaran; Zsuzsanna Nemeth; Anna Safar; Maria Dusinska; Andrew Collins; Diana Anderson; Vanessa Andrade; Cristiana Costa Pereira; Solange Costa

doi:10.1038/s41598-021-95976-7

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Stefano Bonassi^*, Marcello Ceppi, Peter Møller, Amaya Azqueta, Mirta Milić, Neri Monica, Gunnar Brunborg, Roger Godschalk, Gudrun Koppen, Sabine A.S. Langie, João Paulo Teixeira, Marco Bruzzone, Juliana Da Silva, Danieli Benedetti, Delia Cavallo, Cinzia Lucia Ursini, Lisa Giovannelli, Silvia Moretti, Patrizia Riso, Cristian Del Bo’Patrizia Russo, Malgorzata Dobrzyńska, Irina A. Goroshinskaya, Ekaterina I. Surikova, Marta Staruchova, Magdalena Barančokova, Katarina Volkovova, Alena Kažimirova, Bozena Smolkova, Blanca Laffon, Vanessa Valdiglesias, Susana Pastor, Ricard Marcos, Alba Hernández, Goran Gajski, Biljana Spremo-Potparević, Lada Živković, Elisa Boutet-Robinet, Hervé Perdry, Pierre Lebailly, Carlos L. Perez, Nursen Basaran, Zsuzsanna Nemeth, Anna Safar, Maria Dusinska, Andrew Collins, Diana Anderson, Vanessa Andrade, Cristiana Costa Pereira, Solange Costa

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

Original language	English
Article number	16793
Journal	SCIENTIFIC REPORTS
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-95976-7
Publication status	Published - Dec 2021

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Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milić, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., ... Costa, S. (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. SCIENTIFIC REPORTS, 11(1), [16793]. https://doi.org/10.1038/s41598-021-95976-7

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title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death",

abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.",

author = "Stefano Bonassi and Marcello Ceppi and Peter M{\o}ller and Amaya Azqueta and Mirta Mili{\'c} and Neri Monica and Gunnar Brunborg and Roger Godschalk and Gudrun Koppen and Langie, {Sabine A.S.} and Teixeira, {Jo{\~a}o Paulo} and Marco Bruzzone and {Da Silva}, Juliana and Danieli Benedetti and Delia Cavallo and Ursini, {Cinzia Lucia} and Lisa Giovannelli and Silvia Moretti and Patrizia Riso and {Del Bo{\textquoteright}}, Cristian and Patrizia Russo and Malgorzata Dobrzy{\'n}ska and Goroshinskaya, {Irina A.} and Surikova, {Ekaterina I.} and Marta Staruchova and Magdalena Baran{\v c}okova and Katarina Volkovova and Alena Ka{\v z}imirova and Bozena Smolkova and Blanca Laffon and Vanessa Valdiglesias and Susana Pastor and Ricard Marcos and Alba Hern{\'a}ndez and Goran Gajski and Biljana Spremo-Potparevi{\'c} and Lada {\v Z}ivkovi{\'c} and Elisa Boutet-Robinet and Herv{\'e} Perdry and Pierre Lebailly and Perez, {Carlos L.} and Nursen Basaran and Zsuzsanna Nemeth and Anna Safar and Maria Dusinska and Andrew Collins and Diana Anderson and Vanessa Andrade and Pereira, {Cristiana Costa} and Solange Costa",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41598-021-95976-7",

language = "English",

volume = "11",

journal = "Scientific Reports",

issn = "2045-2322",

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Bonassi, S, Ceppi, M, Møller, P, Azqueta, A, Milić, M, Monica, N, Brunborg, G, Godschalk, R, Koppen, G, Langie, SAS, Teixeira, JP, Bruzzone, M, Da Silva, J, Benedetti, D, Cavallo, D, Ursini, CL, Giovannelli, L, Moretti, S, Riso, P, Del Bo’, C, Russo, P, Dobrzyńska, M, Goroshinskaya, IA, Surikova, EI, Staruchova, M, Barančokova, M, Volkovova, K, Kažimirova, A, Smolkova, B, Laffon, B, Valdiglesias, V, Pastor, S, Marcos, R, Hernández, A, Gajski, G, Spremo-Potparević, B, Živković, L, Boutet-Robinet, E, Perdry, H, Lebailly, P, Perez, CL, Basaran, N, Nemeth, Z, Safar, A, Dusinska, M, Collins, A, Anderson, D, Andrade, V, Pereira, CC & Costa, S 2021, 'DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death', SCIENTIFIC REPORTS, vol. 11, no. 1, 16793. https://doi.org/10.1038/s41598-021-95976-7

TY - JOUR

T1 - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

AU - Bonassi, Stefano

AU - Ceppi, Marcello

AU - Møller, Peter

AU - Azqueta, Amaya

AU - Milić, Mirta

AU - Monica, Neri

AU - Brunborg, Gunnar

AU - Godschalk, Roger

AU - Koppen, Gudrun

AU - Langie, Sabine A.S.

AU - Teixeira, João Paulo

AU - Bruzzone, Marco

AU - Da Silva, Juliana

AU - Benedetti, Danieli

AU - Cavallo, Delia

AU - Ursini, Cinzia Lucia

AU - Giovannelli, Lisa

AU - Moretti, Silvia

AU - Riso, Patrizia

AU - Del Bo’, Cristian

AU - Russo, Patrizia

AU - Dobrzyńska, Malgorzata

AU - Goroshinskaya, Irina A.

AU - Surikova, Ekaterina I.

AU - Staruchova, Marta

AU - Barančokova, Magdalena

AU - Volkovova, Katarina

AU - Kažimirova, Alena

AU - Smolkova, Bozena

AU - Laffon, Blanca

AU - Valdiglesias, Vanessa

AU - Pastor, Susana

AU - Marcos, Ricard

AU - Hernández, Alba

AU - Gajski, Goran

AU - Spremo-Potparević, Biljana

AU - Živković, Lada

AU - Boutet-Robinet, Elisa

AU - Perdry, Hervé

AU - Lebailly, Pierre

AU - Perez, Carlos L.

AU - Basaran, Nursen

AU - Nemeth, Zsuzsanna

AU - Safar, Anna

AU - Dusinska, Maria

AU - Collins, Andrew

AU - Anderson, Diana

AU - Andrade, Vanessa

AU - Pereira, Cristiana Costa

AU - Costa, Solange

PY - 2021/12

Y1 - 2021/12

N2 - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

AB - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

UR - http://www.scopus.com/inward/record.url?scp=85113162489&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-95976-7

DO - 10.1038/s41598-021-95976-7

M3 - Article

C2 - 34408182

AN - SCOPUS:85113162489

SN - 2045-2322

VL - 11

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 16793

ER -

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

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