DNA binding studies of a series of cis-[Pt(Am)<inf>2</inf>X<inf>2</inf>] complexes (Am = inert amine, X = labile carboxylato ligand)

Esther Escribano, Mercè Font-Bardia, Teresa Calvet, Julia Lorenzo, Patrick Gamez, Virtudes Moreno

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14 Citations (Scopus)

Abstract

A series of platinum(II) complexes of formulae cis-[Pt(Am) 2X2] (where Am represents an inert amine and X a labile (carboxylato) ligand) have been prepared and characterized by elemental analysis, ESI-MS, IR and 1H NMR spectroscopy. The single-crystal molecular structures were determined for cis-[Pt(opea)(cbdca-2H)], cis-[Pt(hmpy)(cbdca-2H)], cis-[Pt(NH3)2(bzmal-2H)] and cis-[Pt(hmpy)(μ-dcch-2H)2] (where opea is picolylamine, hmpy represents 4-hydroxymethylpyridine, cbdca-2H, is 1,1-cyclobutanedicarboxylate anion, bzmal-2H stands for benzylmalonate anion and dcch-2H is trans-1,2-cyclohexanedicarboxylate anion). The interaction of all compounds with DNA was investigated with different techniques: viscosity measurements and emission fluorescence spectroscopy were used to investigate the changes induced by the binding of the platinum compounds to calf-thymus DNA, while atomic force microscopy and electrophoretic mobility allowed evaluating the potential alterations of pBR322 plasmid DNA. The cytotoxic behavior of the platinum compounds on human leukemia HL-60 tumor cell lines was also examined. © 2012 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)65-76
JournalInorganica Chimica Acta
Volume394
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

  • Atomic force microscopy
  • DNA binding
  • Platinum dicarboxylate compounds
  • Platinum drugs

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