Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds

M. J. Vilar; B. Peralta; I. García-Bocanegra; M. Simon-Grifé; A. Bensaid; J. Casal; J. Segalés; S. Pina-Pedrero

doi:https://doi.org/10.1016/j.virusres.2016.01.019

Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds

M. J. Vilar, B. Peralta, I. García-Bocanegra, M. Simon-Grifé, A. Bensaid, J. Casal, J. Segalés, S. Pina-Pedrero

Department of Animal Health and Anatomy

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

© 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.

Original language	English
Pages (from-to)	42-49
Journal	Virus Research
Volume	215
DOIs	https://doi.org/10.1016/j.virusres.2016.01.019
Publication status	Published - 2 Apr 2016

Keywords

PCR
Phylogenetic analysis
Quasispecies

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@article{c380a0552269449bbf6b2f33a9154ce3,

title = "Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds",

abstract = "{\textcopyright} 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.",

keywords = "PCR, Phylogenetic analysis, Quasispecies",

author = "Vilar, {M. J.} and B. Peralta and I. Garc{\'i}a-Bocanegra and M. Simon-Grif{\'e} and A. Bensaid and J. Casal and J. Segal{\'e}s and S. Pina-Pedrero",

year = "2016",

month = apr,

day = "2",

doi = "https://doi.org/10.1016/j.virusres.2016.01.019",

language = "English",

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pages = "42--49",

journal = "Virus Research",

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TY - JOUR

T1 - Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds

AU - Vilar, M. J.

AU - Peralta, B.

AU - García-Bocanegra, I.

AU - Simon-Grifé, M.

AU - Bensaid, A.

AU - Casal, J.

AU - Segalés, J.

AU - Pina-Pedrero, S.

PY - 2016/4/2

Y1 - 2016/4/2

N2 - © 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.

AB - © 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.

KW - PCR

KW - Phylogenetic analysis

KW - Quasispecies

U2 - https://doi.org/10.1016/j.virusres.2016.01.019

DO - https://doi.org/10.1016/j.virusres.2016.01.019

M3 - Article

VL - 215

SP - 42

EP - 49

JO - Virus Research

JF - Virus Research

SN - 0168-1702

ER -