TY - JOUR
T1 - Distribution and genetic characterization of Enterovirus G and Sapelovirus A in six Spanish swine herds
AU - Vilar, M. J.
AU - Peralta, B.
AU - García-Bocanegra, I.
AU - Simon-Grifé, M.
AU - Bensaid, A.
AU - Casal, J.
AU - Segalés, J.
AU - Pina-Pedrero, S.
PY - 2016/4/2
Y1 - 2016/4/2
N2 - © 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.
AB - © 2016. The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.
KW - PCR
KW - Phylogenetic analysis
KW - Quasispecies
U2 - https://doi.org/10.1016/j.virusres.2016.01.019
DO - https://doi.org/10.1016/j.virusres.2016.01.019
M3 - Article
VL - 215
SP - 42
EP - 49
JO - Virus Research
JF - Virus Research
SN - 0168-1702
ER -