Discriminating the origin of calcium oxalate monohydrate formation in kidney stones via synchrotron microdiffraction

Iris de la Trinidad Henriquez Valido, Victor Fuentes- Cebrian, Roberto Boada Romero, Oriol Vallcorba, Montserrat Resina Gallego, Manuel Valiente Malmagro, Montserrat Lopez Mesas*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Nephrolithiasis is a multifactor disease that produces nephrolites in the kidneys. Calcium oxalate hydrate (dihydrated, COD, or monohydrated, COM) stones are the most common ones with more than sixty percent incidence worldwide. They are related to different pathologies, COD with hypercalciuria and COM with hyperoxaluria. COD is an unstable species and transforms into COM (herein named TRA to distinguish the origin of the monohydrated species). TRA and COM are chemically and crystallographically identical leading to misdiagnosis and recurrence increase. In the current study, the composition and crystalline structures of several calcium oxalate stones, classified by morpho-constitutional analysis, were examined by IR and synchrotron through-the-substrate micro-X-ray diffraction (tts-μXRD). Both IR and linear diffractogram studies were able to distinguish between the monohydrated and dihydrated phases but not between COM and TRA, as expected. The analysis of 2D diffraction patterns revealed that TRA showed a lower degree of crystallinity and less texture with respect to COM which can be used as a signature to distinguish between the two. This study confirms that despite the subtle differences between COM and TRA, the origin of the monohydrate oxalates can be unraveled using tts-μXRD. This valuable information should be taken into account in order to improve patients’ diagnosis and reduce recurrence by considering and treating the origin of the formed stones.
Original languageEnglish
Pages (from-to)349-357
Number of pages9
JournalThe Analyst
Volume147
Issue number2
Early online date6 Dec 2021
DOIs
Publication statusPublished - 21 Jan 2022

Keywords

  • Calcium Oxalate
  • Humans
  • Kidney Calculi
  • Oxalates
  • Synchrotrons
  • X-Ray Diffraction

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