Discriminant biomarkers of acute respiratory distress syndrome associated to H1N1 influenza identified by metabolomics HPLC-QTOF-MS/MS platform

Alessia Ferrarini, Laura Righetti, Ma Paz Martínez, Mariano Fernández-López, Annalaura Mastrangelo, Juan P. Horcajada, Antoni Betbesé, Andrés Esteban, Jordi Ordóñez, Joaquín Gea, Jesús Ruiz Cabello, Federica Pellati, José A. Lorente, Nicolás Nin, Francisco J. Rupérez

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Acute respiratory distress syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis is unknown and biomarkers are lacking. Untargeted metabolomics allows the analysis of the whole metabolome in a biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define the metabolic alterations occurring in patients with influenza A (H1N1) virus infection that developed ARDS. Serum samples from patients diagnosed with 2009 influenza A (H1N1) virus infection with (n = 25) or without (n = 32) ARDS were obtained on the day of hospital admission and analyzed by LC-MS/MS. Metabolite identification was determined by MS/MS analysis and analysis of standards. The specificity of the patterns identified was confirmed in patients without 2009 influenza A(H1N1) virus pneumonia (15 without and 17 with ARDS). Twenty-three candidate biomarkers were found to be significantly different between the two groups, including lysophospholipids and sphingolipids related to inflammation; bile acids, tryptophan metabolites, and thyroxine, related to the metabolism of the gut microflora. Confirmation results demonstrated the specificity of major alterations occurring in ARDS patients with influenza A (H1N1) virus infection.
Original languageEnglish
Pages (from-to)2341-2348
JournalElectrophoresis
Volume38
Issue number18
DOIs
Publication statusPublished - 1 Sep 2017

Keywords

  • ARDS
  • Biomarkers
  • Influenza A (H1N1) virus infection
  • Pathogenesis
  • Untargeted metabolomics

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