Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site

Victor A. Streltsov, Sukanya Luang, Alys Peisley, Joseph N. Varghese, James R. Ketudat Cairns, Sebastien Fort, Marcel Hijnen, Igor Tvaroška, Ana Ardá, Jesús Jiménez-Barbero, Mercedes Alfonso-Prieto, Carme Rovira, Fernanda Mendoza, Laura Tiessler-Sala, José Emilio Sánchez-Aparicio, Jaime Rodríguez-Guerra, José M. Lluch, Jean Didier Maréchal, Laura Masgrau, Maria Hrmova

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Substrates associate and products dissociate from enzyme catalytic sites rapidly, which hampers investigations of their trajectories. The high-resolution structure of the native Hordeum exo-hydrolase HvExol isolated from seedlings reveals that non-covalently trapped glucose forms a stable enzyme-product complex. Here, we report that the alkyl beta-D-glucoside and methyl 6-thio-beta-gentiobioside substrate analogues perfused in crystalline HvExol bind across the catalytic site after they displace glucose, while methyl 2-thio-beta-sophoroside attaches nearby. Structural analyses and multi-scale molecular modelling of nanoscale reactant movements in HvExol reveal that upon productive binding of incoming substrates, the glucose product modifies its binding patterns and evokes the formation of a transient lateral cavity, which serves as a conduit for glucose departure to allow for the next catalytic round. This path enables substrate-product assisted processive catalysis through multiple hydrolytic events without HvExol losing contact with oligo- or polymeric substrates. We anticipate that such enzyme plasticity could be prevalent among exo-hydrolases.

Original languageEnglish
Article number2222
Pages (from-to)2222
Number of pages17
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Biocatalysis
  • Catalytic Domain
  • Crystallography, X-Ray
  • Enzyme Assays/methods
  • Glucosidases/chemistry
  • Glycosides/metabolism
  • Hordeum/metabolism
  • Models, Molecular
  • Molecular Dynamics Simulation
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Proteins/chemistry
  • Recombinant Proteins/isolation & purification
  • Seedlings/metabolism
  • Substrate Specificity

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