Discovering putative prion-like proteins in Plasmodium falciparum: A computational and experimental analysis

Irantzu Pallarès, Natalia S. de Groot, Valentín Iglesias, Ricardo Sant'Anna, Arnau Biosca, Xavier Fernàndez-Busquets, Salvador Ventura

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31 Citations (Scopus)


© 2018 Pallarès, de Groot, Iglesias, Sant'Anna, Biosca, Fernàndez-Busquets and Ventura. Prions are a singular subset of proteins able to switch between a soluble conformation and a self-perpetuating amyloid state. Traditionally associated with neurodegenerative diseases, increasing evidence indicates that organisms exploit prion-like mechanisms for beneficial purposes. The ability to transit between conformations is encoded in the so-called prion domains, long disordered regions usually enriched in glutamine/asparagine residues. Interestingly, Plasmodium falciparum, the parasite that causes the most virulent form of malaria, is exceptionally rich in proteins bearing long Q/N-rich sequence stretches, accounting for roughly 30% of the proteome. This biased composition suggests that these protein regions might correspond to prion-like domains (PrLDs) and potentially form amyloid assemblies. To investigate this possibility, we performed a stringent computational survey for Q/N-rich PrLDs on P. falciparum. Our data indicate that ~10% of P. falciparum protein sequences have prionic signatures, and that this subproteome is enriched in regulatory proteins, such as transcription factors and RNA-binding proteins. Furthermore, we experimentally demonstrate for several of the identified PrLDs that, despite their disordered nature, they contain inner short sequences able to spontaneously self-assemble into amyloid-like structures. Although the ability of these sequences to nucleate the conformational conversion of the respective full-length proteins should still be demonstrated, our analysis suggests that, as previously described for other organisms, prion-like proteins might also play a functional role in P. falciparum.
Original languageEnglish
Article number1737
JournalFrontiers in Microbiology
Issue numberAUG
Publication statusPublished - 7 Aug 2018


  • Amyloid
  • Plasmodium
  • Prion
  • Protein aggregation
  • Protein disorder
  • Q/N-rich sequences


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