TY - JOUR
T1 - Directed self-assembly of trimeric DNA-bindingchiral miniprotein helicates
AU - Gómez-González, Jacobo
AU - Peña, Diego G.
AU - Barka, Ghofrane
AU - Sciortino, Giuseppe
AU - Maréchal, Jean Didier
AU - López, Miguel Vázquez
AU - Vázquez, M. Eugenio
PY - 2018/10/1
Y1 - 2018/10/1
N2 - © 2018 Gómez-González, Peña, Barka, Sciortino, Maréchal, Vázquez López and Vázquez. We propose that peptides are highly versatile platforms for the precise design of supramolecular metal architectures, and particularly, for the controlled assembly of helicates. In this context, we show that the bacteriophage T4 Fibritin foldon (T4Ff) can been engineered on its N-terminus with metal-chelating 2,2'-bipyridine units that stereoselectively assemble in the presence of Fe(II) into parallel, three-stranded peptide helicates with preferred helical orientation. Modeling studies support the proposed self-assembly and the stability of the final helicate. Furthermore, we show that these designed mini-metalloproteins selectively recognize three-way DNA junctions over double-stranded DNA.
AB - © 2018 Gómez-González, Peña, Barka, Sciortino, Maréchal, Vázquez López and Vázquez. We propose that peptides are highly versatile platforms for the precise design of supramolecular metal architectures, and particularly, for the controlled assembly of helicates. In this context, we show that the bacteriophage T4 Fibritin foldon (T4Ff) can been engineered on its N-terminus with metal-chelating 2,2'-bipyridine units that stereoselectively assemble in the presence of Fe(II) into parallel, three-stranded peptide helicates with preferred helical orientation. Modeling studies support the proposed self-assembly and the stability of the final helicate. Furthermore, we show that these designed mini-metalloproteins selectively recognize three-way DNA junctions over double-stranded DNA.
KW - Coordination chemistry
KW - DNA recognition
KW - Enantioselectivity
KW - Metallopeptide
KW - Peptide motifs
KW - Self-assembly water
U2 - https://doi.org/10.3389/fchem.2018.00520
DO - https://doi.org/10.3389/fchem.2018.00520
M3 - Article
C2 - 30425980
VL - 6
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
SN - 2296-2646
M1 - 520
ER -