Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle

Dionisia Fontanals; Alex Larruzea; Isabel Sanfeliu

doi:10.1016/j.anaerobe.2018.07.009

Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle

Dionisia Fontanals, Alex Larruzea, Isabel Sanfeliu

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research

4 Citations (Scopus)

Abstract

© 2018 Elsevier Ltd We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram staining of positive anaerobic blood cultures revealed both diplococci and short chains of gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.

Original language	English
Pages (from-to)	264-266
Journal	Anaerobe
Volume	54
DOIs	https://doi.org/10.1016/j.anaerobe.2018.07.009
Publication status	Published - 1 Dec 2018

Keywords

Bloodstream infection
MALDI-TOF MS
Ruminococcus gnavus

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/j.anaerobe.2018.07.009

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title = "Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle",

abstract = "{\textcopyright} 2018 Elsevier Ltd We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram staining of positive anaerobic blood cultures revealed both diplococci and short chains of gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.",

keywords = "Bloodstream infection, MALDI-TOF MS, Ruminococcus gnavus",

author = "Dionisia Fontanals and Alex Larruzea and Isabel Sanfeliu",

year = "2018",

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doi = "10.1016/j.anaerobe.2018.07.009",

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T1 - Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle

AU - Fontanals, Dionisia

AU - Larruzea, Alex

AU - Sanfeliu, Isabel

PY - 2018/12/1

Y1 - 2018/12/1

N2 - © 2018 Elsevier Ltd We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram staining of positive anaerobic blood cultures revealed both diplococci and short chains of gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.

AB - © 2018 Elsevier Ltd We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram staining of positive anaerobic blood cultures revealed both diplococci and short chains of gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.

KW - Bloodstream infection

KW - MALDI-TOF MS

KW - Ruminococcus gnavus

U2 - 10.1016/j.anaerobe.2018.07.009

DO - 10.1016/j.anaerobe.2018.07.009

M3 - Article

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VL - 54

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EP - 266

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

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