Differential role of tumor necrosis factor receptors in mouse brain inflammatory responses in cryolesion brain injury

Albert Quintana, Mercedes Giralt, Santiago Rojas, Milena Penkowa, Iain L. Campbell, Juan Hidalgo, Amalia Molinero

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNF-α) is one of the mediators dramatically increased after traumatic brain injury that leads to the activation, proliferation, and hypertrophy of mononuclear, phagocytic cells and gliosis. Eventually, TNF-α can induce both apoptosis and necrosis via intracellular signaling. This cytokine exerts its functions via interaction with two receptors: type-1 receptor (TNFR1) and type-2 receptor (TNFR2). In this work, the inflammatory response after a freeze injury (cryolesion) in the cortex was studied in wild-type (WT) animals and in mice lacking TNFR1 (TNFR1 KO) or TNFR2 (TNFR2 KO). Lack of TNFR1, but not of TNFR2, significantly decreased the inflammatory response and tissue damage elicited by the cryolesion at both 3 and 7 days postlesion, with decreased gliosis, lower IL-1β immunostaining, and a reduction of apoptosis markers. Cryolesion produced a clear induction of the proinflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6, and TNF-α; this induction was significantly lower in the TNFR1 KO mice. Host response genes (ICAM-1, A20, EB22/5, and GFAP) were also induced by the cryolesion, but to a lesser extent in TNFR1 KO mice. Lack of TNFR1 signaling also affected the expression of apoptosis/cell death-related genes (Fas, Rip, p53), matrix metalloproteinases (MMP3, MMP9, MMP12), and their inhibitors (TIMP1), suggesting a role of TNFR1 in extracellular matrix remodeling after injury. However, GDNF, NGF, and BDNF expression were not affected by TNFR1 deficiency. Overall, these results suggest that TNFR1 is involved in the early establishment of the inflammatory response and that its deficiency causes a decreased inflammatory response and tissue damage following brain injury. © 2005 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)701-716
JournalJournal of Neuroscience Research
Volume82
DOIs
Publication statusPublished - 1 Dec 2005

Keywords

  • Apoptosis
  • Gene expression
  • Knockout type-1 TNF receptor
  • Knockout type-2 TNF receptor
  • Traumatic brain injury

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