Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats

Marta Sabariego, M. José Gómez, Ignacio Morón, Carmen Torres, Alberto Fernández-Teruel, Adolfo Tobeña, Toni Cañete, José A. Martínez-Conejero, José A. Horcajadas, Francisco J. Esteban

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16 Citations (Scopus)

Abstract

Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change. © 2011.
Original languageEnglish
Pages (from-to)265-270
JournalNeuroscience Letters
Volume504
Issue number3
DOIs
Publication statusPublished - 31 Oct 2011

Keywords

  • Anxiety
  • CDNA microarray
  • Gene expression
  • Roman rats
  • RT-PCR
  • Two-way avoidance acquisition

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