Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats

Marta Sabariego; M. José Gómez; Ignacio Morón; Carmen Torres; Alberto Fernández-Teruel; Adolfo Tobeña; Toni Cañete; José A. Martínez-Conejero; José A. Horcajadas; Francisco J. Esteban

doi:10.1016/j.neulet.2011.09.044

Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats

Marta Sabariego, M. José Gómez, Ignacio Morón, Carmen Torres, Alberto Fernández-Teruel, Adolfo Tobeña, Toni Cañete, José A. Martínez-Conejero, José A. Horcajadas, Francisco J. Esteban

Research output: Contribution to journal › Article › Research › peer-review

16 Citations (Scopus)

Abstract

Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change. © 2011.

Original language	English
Pages (from-to)	265-270
Journal	Neuroscience Letters
Volume	504
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2011.09.044
Publication status	Published - 31 Oct 2011

Keywords

Anxiety
CDNA microarray
Gene expression
Roman rats
RT-PCR
Two-way avoidance acquisition

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Sabariego, Marta ; Gómez, M. José ; Morón, Ignacio ; Torres, Carmen ; Fernández-Teruel, Alberto ; Tobeña, Adolfo ; Cañete, Toni ; Martínez-Conejero, José A. ; Horcajadas, José A. ; Esteban, Francisco J. / Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats. In: Neuroscience Letters. 2011 ; Vol. 504, No. 3. pp. 265-270.

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title = "Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats",

abstract = "Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change. {\textcopyright} 2011.",

keywords = "Anxiety, CDNA microarray, Gene expression, Roman rats, RT-PCR, Two-way avoidance acquisition",

author = "Marta Sabariego and G{\'o}mez, {M. Jos{\'e}} and Ignacio Mor{\'o}n and Carmen Torres and Alberto Fern{\'a}ndez-Teruel and Adolfo Tobe{\~n}a and Toni Ca{\~n}ete and Mart{\'i}nez-Conejero, {Jos{\'e} A.} and Horcajadas, {Jos{\'e} A.} and Esteban, {Francisco J.}",

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Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats. / Sabariego, Marta; Gómez, M. José; Morón, Ignacio; Torres, Carmen; Fernández-Teruel, Alberto ; Tobeña, Adolfo; Cañete, Toni; Martínez-Conejero, José A.; Horcajadas, José A.; Esteban, Francisco J.

In: Neuroscience Letters, Vol. 504, No. 3, 31.10.2011, p. 265-270.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats

AU - Sabariego, Marta

AU - Gómez, M. José

AU - Morón, Ignacio

AU - Torres, Carmen

AU - Fernández-Teruel, Alberto

AU - Tobeña, Adolfo

AU - Cañete, Toni

AU - Martínez-Conejero, José A.

AU - Horcajadas, José A.

AU - Esteban, Francisco J.

PY - 2011/10/31

Y1 - 2011/10/31

N2 - Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change. © 2011.

AB - Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change. © 2011.

KW - Anxiety

KW - CDNA microarray

KW - Gene expression

KW - Roman rats

KW - RT-PCR

KW - Two-way avoidance acquisition

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DO - 10.1016/j.neulet.2011.09.044

M3 - Article

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