Abstract
The effect of transforming growth factor β (TGF-β) on the biosynthesis of individual proteoglycans (PGs) by human embryonic lung fibroblasts has been investigated using specific antibodies and cDNA probes. Human lung fibroblasts secrete the two small chondrotin/dermatan sulfate PGs, PG-I or biglycan (300 kDA) and, in a larger proportion, PG-II or decorin (130 kDa). Metabolic labeling experiments reveal that TGF-β induces selectivity the expression of PG-I, whereas the level of PG-II remains unaltered. The effect of TGF-β on PG-I and PG-II has been studied by immunoprecipitation and Northern blot analysis. Either at the core protein or mRNA level, a specific 5-fold increase in PG-I can be observed. TGF-β acts probably at the transcriptional level, as actinomycin D blocks completely the TGF-β induced proteoglycan synthesis. A low saturation density and a slower growth rate is also observed for TGF-β treated cells. The possible role of PG-I and PG-II as mediators of the growth inhibition caused by TGF-β is discussed. © 1991.
| Original language | English |
|---|---|
| Pages (from-to) | 229-233 |
| Journal | BBA - Molecular Cell Research |
| Volume | 1093 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 10 Jul 1991 |
Keywords
- (Human embryonic lung fibroblast)
- Proteoglycan synthesis
- Transforming growth factor
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Romarís, M., Heredia, A., Molist, A., & Bassols, A. (1991). Differential effect of transforming growth factor β on proteoglycan synthesis in human embryonic lung fibroblasts. BBA - Molecular Cell Research, 1093(2-3), 229-233. https://doi.org/10.1016/0167-4889(91)90127-J