Different α<inf>1</inf>-adrenoceptor-induced inositol phosphate formation in the two portions of rat vas deferens

D. Terradas, Antonia Tabernero, A. Badia, N. M. Vivas

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The two portions of rat vas deferens differed in the postjunctional sensitivity to noradrenaline. α1-Adrenoceptor-linked phosphoinositide breakdown was analysed in this tissue. The noradrenaline-induced [3H]inositol phosphate accumulation was similar in both ends although the pEC50 was higher in the epididymal (5.97±0.07) than in the prostatic (5.47±0.15, P>0.01) portion. [3H]Prazosin showed similar density of binding sites in both portions. Tissue pretreated with pertussis toxin did not change [3H]inositol phosphate accumulation. Finally, Western blot analysis indicated a smaller concentration of Gq/11 protein in the prostatic half (-29±5%, P>0.01). These results suggest that the different sensitivity to noradrenaline could be due to the higher availability of this sort of G protein in the epididymal portion.
Original languageEnglish
Pages (from-to)11-15
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume363
Issue number1
DOIs
Publication statusPublished - 1 Jan 2001

Keywords

  • G protein
  • Inositol phosphates
  • Pertussis toxin
  • [ H]Prazosin binding 3
  • α -Adrenoceptor 1

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