Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics

Ivan Erill, Mónica Jara, Noelia Salvador, Marcos Escribano, Susana Campoy, Jordi Barbé

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)

Abstract

The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes (recA, ssb, uvrA and ruvCAB) might be a defining property of the Proteobacteria LexA network. © Oxford University Press 2004; all rights reserved.
Original languageEnglish
Pages (from-to)6617-6626
JournalNucleic Acids Research
Volume32
DOIs
Publication statusPublished - 1 Dec 2004

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