Diagnostic yield of transbronchial cryobiopsy in interstitial lung disease: A randomized trial

Virginia Pajares, Carmen Puzo, Diego Castillo, Enrique Lerma, M. Angeles Montero, David Ramos-Barbõn, Oscar Amor-Carro, Angels Gil De Bernabé, Tomás Franquet, Vicente Plaza, Jürgen Hetzel, Joaquin Sanchis, Alfons Torrego

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194 Citations (Scopus)


Background and objective Transbronchial lung biopsy (TBLB) is required for evaluation in selected patients with interstitial lung disease (ILD). The diagnostic yield of histopathologic assessment is variable and is influenced by factors such as the size of samples and the presence of crush artefacts left by conventional biopsy forceps. We compared the diagnostic yield and safety of TBLB with cryoprobe sampling versus conventional forceps sampling. Methods This randomized clinical trial analysed data for 77 patients undergoing TBLB for evaluation of ILD; patients were assigned to either a conventional-forceps group or a cryoprobe group. Two pathologists assessed the tissue samples and agreed on histopathologic diagnoses. We also compared the duration of procedures, complications and sample-quality variables. Results The most frequent diagnosis observed in the cryoprobe group was non-specific interstitial pneumonia. Histopathologic diagnoses were identified in more cases in the cryoprobe group (74.4%) than in the conventional-forceps group (34.1%) (P < 0.001), and the diagnostic yield was higher in the cryoprobe group (51.3% vs 29.1% in the conventional forceps group; P = 0.038). A larger mean area of tissue was harvested by cryoprobe (14.7 ± 11 mm2) than by conventional forceps (3.3 ± 4.1 mm2) (P < 0.001). More grade 2 bleeding (not statistically significant) occurred in the cryoprobe group (56.4%) than in the conventional-forceps group (34.2%). No differences in other complications were observed. Conclusions TBLB by cryoprobe is safe and potentially useful in the diagnosis of ILD. Larger multisite randomized trials are required to confirm the potential benefits of this procedure. Clinical trial registration at ClinicalTrials.gov: NCT01064609. The diagnostic yield of transbronchial lung biopsy in interstitial lung disease is variable. This is the first randomized trial to demonstrate that the yield of transbronchial lung biopsy is higher with cryoprobes than with conventional forceps in patients with interstitial lung diseases requiring a bronchoscopic procedure for diagnosis. See Editorial, page 773 © 2014 Asian Pacific Society of Respirology.
Original languageEnglish
Pages (from-to)900-906
Publication statusPublished - 1 Jan 2014


  • bronchoscopy
  • cryobiopsy
  • cryoprobe
  • interstitial lung disease
  • transbronchial lung biopsy


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