Diagnosis of canine leishmaniasis by a polymerase chain reaction technique

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    Abstract

    A polymerase chain reaction (PCR) technique for the diagnosis of canine leishmaniasis on bone marrow samples was developed which amplified a 120 bp DNA fragment of the Leishmania kinetoplast DNA, common to all Leishmania species. Forty-five of 46 dogs in which leishmaniasis had been diagnosed were positive with the PCR technique, whereas none of 41 healthy dogs gave a positive result. Fifteen dogs with leishmaniasis that had been treated for six months with N-methylglucamine antimoniate and allopurinol were also investigated. Seven were positive, implying that they remained infected despite the resolution of their clinical signs.
    Original languageEnglish
    Pages (from-to)262-264
    JournalVeterinary Record
    Volume144
    Issue number10
    DOIs
    Publication statusPublished - 6 Mar 1999

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