Diagnosis of avian adenovirus infections using DNA in situ hybridization

Kenneth S. Latimer, Frank D. Niagro, Orlando C. Williams, Antonio Ramis, Mark A. Goodwin, Branson W. Ritchie, Raymond P. Campagnoli

    Research output: Contribution to journalArticleResearchpeer-review

    21 Citations (Scopus)

    Abstract

    Three DNA oligonucleotide probes designated FN-23, FN-48, and FN-96 were evaluated for the diagnosis of aviadenovirus infections by DNA in situ hybridization. Paraffin-embedded tissues were obtained from birds with confirmed adenovirus infection, birds with putative adenovirus infections, and birds with intranuclear inclusions caused by herpesvirus and polyomavirus. In birds with confirmed adenovirus infection, probes FN-23 and FN-96 identified 78% and 72% of diseased individuals, respectively. Only probe FN-48 detected chickens with group II adenovirus infection. In birds with putative adenovirus infection, the DNA probes confirmed aviadenovirus infections in 76% of the population. Probes FN-23, FN-96, and FN-48 detected 85%, 74%, and 18% of adenovirus-infected birds, respectively. None of the DNA probes cross-hybridized with tissues from polyomavirus-infected psittaciform birds or with tissues from a chicken with infectious laryngotracheitis. In contrast, probe FN-23 did cross-hybridize to herpesvirus-infected tissues from two of eight psittaciform birds with Pacheco's parrot disease. Probes FN-48 and FN-96 did not react with these tissues.
    Original languageEnglish
    Pages (from-to)773-782
    JournalAvian Diseases
    Volume41
    Issue number4
    DOIs
    Publication statusPublished - 1 Jan 1997

    Keywords

    • Aviadenovirus
    • Avian adenovirus
    • Chicken
    • Herpesvirus
    • In situ hybridization
    • Pacheco's parrot disease
    • Polyomavirus
    • Psittaciform birds

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