The design and construction of a sequential microtitration system are reported in the present work. The combination of the sequential injection analysis technique and a reaction chamber is the basis of this novel system. The reaction chamber features an integrated sensing assembly with miniature all-solid-state sensors. This setup is operated easily using titrant and sample solutions in the microliter range, much lower than the volumes employed in automatic titration systems. Furthermore, a separate reaction vessel for each sample is not required in the system reported here. The proposed setup titrates using sequential injections and does not need calibration procedures, as often happens with other pseudotitration systems adapted to flow systems. The sequential microtitration system has been applied to a wide range of acid-base, complexometric, precipitation, and redox titrations. Accuracy and reproducibility of the method proposed are comparable to those obtained in batch titrations. The typical analysis time is around 5 min. The time employed in a cleaning operation or in a sample change procedure is under 2 min.
|Publication status||Published - 15 Apr 1996|