TY - JOUR
T1 - Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces
AU - Ripolles-Avila, Carolina
AU - Ríos-Castillo, Abel G.
AU - Rodríguez-Jerez, José J.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - © 2018 The Author(s). Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis.With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 104 CFU cm−2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.
AB - © 2018 The Author(s). Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis.With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 104 CFU cm−2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.
KW - Biodetector
KW - Biofilms
KW - Detection
KW - Food hygiene
KW - Rapid method
U2 - 10.1080/19476337.2017.1418434
DO - 10.1080/19476337.2017.1418434
M3 - Article
VL - 16
SP - 506
EP - 515
JO - CYTA - Journal of Food
JF - CYTA - Journal of Food
SN - 1947-6337
IS - 1
ER -