Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

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Abstract

© 2018 The Author(s). Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis.With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 104 CFU cm−2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.
Original languageEnglish
Pages (from-to)506-515
JournalCYTA - Journal of Food
Volume16
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Biodetector
  • Biofilms
  • Detection
  • Food hygiene
  • Rapid method

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