Development and validation of a gas chromatography-mass spectrometry assay for hair analysis of amphetamine, methamphetamine and methylenedioxy derivatives

Mitona Pujadas, Simona Pichini, Sandra Poudevida, Ester Menoyo, Piergiorgio Zuccaro, Magí Farré, Rafael De La Torre

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54 Citations (Scopus)

Abstract

A procedure based on gas chromatography-mass spectrometry (GC-MS) is described for the determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA, ecstasy), 3,4-methylenedioxyethylamphetamine (MDE or MDEA) and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in hair. Hair samples were digested with 1M sodium sulfide at 37°C (by shaking for 3h and was kept at room temperature overnight), and extracted with two sequential extraction procedures: liquid-liquid extraction with tert-butyl methyl ether and solid-phase extraction with Bond-Elut Certify columns. Extracted analytes were derivatised with N-methyl-bis(trifluoroacetamide), separated by a 5% phenylmethylsilicone column and determined by a mass spectrometer detector in selected ion monitoring mode. A good reproducibility (intra-assay R.S.D.=1.5-15.7%), accuracy (intra-assay error=2.0-11.7%) and sensitivity (LOD=0.03-0.08ng/mg hair) were attained. The method was successfully applied to the analysis of the proximal (1cm) hair segment to assess recent self-reported use in "ecstasy" consumers. Otherwise, further studies are needed to validate methodology developed in case of amphetamine consumption. © 2003 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)249-255
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume798
DOIs
Publication statusPublished - 25 Dec 2003

Keywords

  • Amphetamine
  • Derivatization, GC
  • Methamphetamine

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