Determination of tryptophan in feed samples by cyclic voltammetry and multivariate calibration methods

Javier Saurina; Santiago Hernández-Cassou; Esteve Fàbregas; Salvador Alegret

doi:10.1039/a901149h

Determination of tryptophan in feed samples by cyclic voltammetry and multivariate calibration methods

Javier Saurina, Santiago Hernández-Cassou, Esteve Fàbregas, Salvador Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

38 Citations (Scopus)

Abstract

A voltammetric method for the determination of tryptophan (Trp) in feed samples using multivariate calibration techniques is proposed. The multivariate data consisted of the oxidation wave of cyclic voltammograms registered from 400 to 925 mV using a graphite-methacrylate composite electrode, at which Trp was oxidized around a potential of 900 mV. The interference from other oxidizable amino acids as well as the feed sample matrix was circumvented by using multivariate calibration methods. Principal component regression (PCR) and partial least squares regression (PLS) and non-linear PLS (NL-PLS) were checked for quantifying Trp in these samples. The results obtained using NL-PLS were in agreement with those obtained with the standard method for the determination of amino acids, with an overall prediction error of 7.9%.

Original language	English
Pages (from-to)	733-737
Journal	The Analyst
Volume	124
Issue number	5
DOIs	https://doi.org/10.1039/a901149h
Publication status	Published - 1 Jan 1999

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title = "Determination of tryptophan in feed samples by cyclic voltammetry and multivariate calibration methods",

abstract = "A voltammetric method for the determination of tryptophan (Trp) in feed samples using multivariate calibration techniques is proposed. The multivariate data consisted of the oxidation wave of cyclic voltammograms registered from 400 to 925 mV using a graphite-methacrylate composite electrode, at which Trp was oxidized around a potential of 900 mV. The interference from other oxidizable amino acids as well as the feed sample matrix was circumvented by using multivariate calibration methods. Principal component regression (PCR) and partial least squares regression (PLS) and non-linear PLS (NL-PLS) were checked for quantifying Trp in these samples. The results obtained using NL-PLS were in agreement with those obtained with the standard method for the determination of amino acids, with an overall prediction error of 7.9%.",

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T1 - Determination of tryptophan in feed samples by cyclic voltammetry and multivariate calibration methods

AU - Saurina, Javier

AU - Hernández-Cassou, Santiago

AU - Fàbregas, Esteve

AU - Alegret, Salvador

PY - 1999/1/1

Y1 - 1999/1/1

N2 - A voltammetric method for the determination of tryptophan (Trp) in feed samples using multivariate calibration techniques is proposed. The multivariate data consisted of the oxidation wave of cyclic voltammograms registered from 400 to 925 mV using a graphite-methacrylate composite electrode, at which Trp was oxidized around a potential of 900 mV. The interference from other oxidizable amino acids as well as the feed sample matrix was circumvented by using multivariate calibration methods. Principal component regression (PCR) and partial least squares regression (PLS) and non-linear PLS (NL-PLS) were checked for quantifying Trp in these samples. The results obtained using NL-PLS were in agreement with those obtained with the standard method for the determination of amino acids, with an overall prediction error of 7.9%.

AB - A voltammetric method for the determination of tryptophan (Trp) in feed samples using multivariate calibration techniques is proposed. The multivariate data consisted of the oxidation wave of cyclic voltammograms registered from 400 to 925 mV using a graphite-methacrylate composite electrode, at which Trp was oxidized around a potential of 900 mV. The interference from other oxidizable amino acids as well as the feed sample matrix was circumvented by using multivariate calibration methods. Principal component regression (PCR) and partial least squares regression (PLS) and non-linear PLS (NL-PLS) were checked for quantifying Trp in these samples. The results obtained using NL-PLS were in agreement with those obtained with the standard method for the determination of amino acids, with an overall prediction error of 7.9%.

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DO - 10.1039/a901149h

M3 - Article

VL - 124

SP - 733

EP - 737

IS - 5

ER -

Determination of tryptophan in feed samples by cyclic voltammetry and multivariate calibration methods

Abstract

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