Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

J. Perelló, B. Isern, J. A. Muñoz, M. Valiente, F. Grases

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9 Citations (Scopus)


An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.
Original languageEnglish
Pages (from-to)265-268
Issue number5-6
Publication statusPublished - 1 Sep 2004


  • Column liquid chromatography
  • Human urine samples
  • Mass spectrometric detection
  • Phytate


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