Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

J. Perelló; B. Isern; J. A. Muñoz; M. Valiente; F. Grases

doi:10.1365/s10337-004-0379-5

Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

J. Perelló, B. Isern, J. A. Muñoz, M. Valiente, F. Grases

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.

Original language	English
Pages (from-to)	265-268
Journal	Chromatographia
Volume	60
Issue number	5-6
DOIs	https://doi.org/10.1365/s10337-004-0379-5
Publication status	Published - 1 Sep 2004

Keywords

Column liquid chromatography
Human urine samples
Mass spectrometric detection
Phytate

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title = "Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry",

abstract = "An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.",

keywords = "Column liquid chromatography, Human urine samples, Mass spectrometric detection, Phytate",

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T1 - Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

AU - Perelló, J.

AU - Isern, B.

AU - Muñoz, J. A.

AU - Valiente, M.

AU - Grases, F.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.

AB - An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.

KW - Column liquid chromatography

KW - Human urine samples

KW - Mass spectrometric detection

KW - Phytate

U2 - 10.1365/s10337-004-0379-5

DO - 10.1365/s10337-004-0379-5

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JO - Chromatographia

JF - Chromatographia

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