An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.
- Column liquid chromatography
- Human urine samples
- Mass spectrometric detection
Perelló, J., Isern, B., Muñoz, J. A., Valiente, M., & Grases, F. (2004). Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry. Chromatographia, 60(5-6), 265-268. https://doi.org/10.1365/s10337-004-0379-5