Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

J. Perelló; B. Isern; J. A. Muñoz; M. Valiente; F. Grases

doi:10.1365/s10337-004-0379-5

Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry

J. Perelló, B. Isern, J. A. Muñoz, M. Valiente, F. Grases

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.

Original language	English
Pages (from-to)	265-268
Journal	Chromatographia
Volume	60
Issue number	5-6
DOIs	https://doi.org/10.1365/s10337-004-0379-5
Publication status	Published - 1 Sep 2004

Keywords

Column liquid chromatography
Human urine samples
Mass spectrometric detection
Phytate

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Perelló, J., Isern, B., Muñoz, J. A., Valiente, M., & Grases, F. (2004). Determination of phytate in urine by high-performance liquid chromatography-mass spectrometry. Chromatographia, 60(5-6), 265-268. https://doi.org/10.1365/s10337-004-0379-5

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abstract = "An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.",

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AU - Grases, F.

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AB - An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z = 198, which corresponds to the adduct of myoinositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L-1 phytate were 4.1, 3.0, and 2.7% respectively (n = 5). The limit of detection was 60 μg L-1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC-MS. The results from both methods were comparable.

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KW - Human urine samples

KW - Mass spectrometric detection

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