Determination of lysine in pharmaceutical samples containing endogenous ammonium ions by using a lysine oxidase biosensor based on an all-solid- state potentiometric ammonium electrode

Javier Saurina, Santiago Hernández-Cassou, Salvador Alegret, Esteve Fàbregas

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

A new potentiometric method is proposed to determine lysine in pharmaceutical samples. This method is based on a lysine biosensor consisting of a chemically immobilized lysine oxidase membrane attached to an all- solid-state ammonium electrode. Lysine is degraded in the sensor to release ammonium, which is detected by means of the ammonium electrode. The presence of endogenous ammonium in the samples interferes with these determinations, since the response measured corresponds to the sum of the ammonium generated enzymatically and that present in the sample. This is a general drawback for all biosensors based on the detection of ammonium. Study of samples containing both lysine and ammonium showed that concentration ranges exist in which a near-logarithmic relationship between potentials measured and lysine concentrations is found. Therefore, within these ranges, lysine can be determined by using the standard addition method, with the subsequent data treatment involving an iterative linearization procedure. Results obtained with the proposed potentiometric method are consistent with those given by the standard method for amino acid analysis.
Original languageEnglish
Pages (from-to)67-75
JournalBiosensors and Bioelectronics
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Jan 1999

Keywords

  • Lysine determination
  • Lysine oxidase
  • Pharmaceutical samples
  • Potentiometric lysine biosensor

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