Determination of indomethacin residues in poultry by high-performance liquid chromatography

C. Cristòfol; B. Pérez; M. Pons; J. E. Valladares; G. Martí; M. Arboix

doi:10.1016/S0378-4347(98)00083-8

Determination of indomethacin residues in poultry by high-performance liquid chromatography

C. Cristòfol, B. Pérez, M. Pons, J. E. Valladares, G. Martí, M. Arboix

Department of Pharmacology, Therapeutics and Toxicology

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.

Original language	English
Pages (from-to)	310-314
Journal	Journal of Chromatography B: Biomedical Applications
Volume	709
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(98)00083-8
Publication status	Published - 29 May 1998

Keywords

Indomethacin

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title = "Determination of indomethacin residues in poultry by high-performance liquid chromatography",

abstract = "A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.",

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AU - Cristòfol, C.

AU - Pérez, B.

AU - Pons, M.

AU - Valladares, J. E.

AU - Martí, G.

AU - Arboix, M.

PY - 1998/5/29

Y1 - 1998/5/29

N2 - A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.

AB - A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.

KW - Indomethacin

U2 - 10.1016/S0378-4347(98)00083-8

DO - 10.1016/S0378-4347(98)00083-8

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SP - 310

EP - 314

IS - 2

ER -