TY - JOUR
T1 - Determination of indomethacin residues in poultry by high-performance liquid chromatography
AU - Cristòfol, C.
AU - Pérez, B.
AU - Pons, M.
AU - Valladares, J. E.
AU - Martí, G.
AU - Arboix, M.
PY - 1998/5/29
Y1 - 1998/5/29
N2 - A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.
AB - A HPLC method using a C18 column and UV detection (254 nm) is described for the determination of indomethacin residues in chicken tissues (liver, muscle and fat). Drug extraction from tissue homogenate in phosphate buffer (pH 3.5) was performed with dichloromethane. Mobile phase was acetonitrile-acetic acid (0.5% in water) (50:50). Indomethacin detection limit was 20 ng/g for the studied tissues. After administration of an oral dose of indomethacin (2 mg/kg), only three of the eight poultry studied showed drug tissue levels, in those cases the levels were below 50 ng/g.
KW - Indomethacin
U2 - 10.1016/S0378-4347(98)00083-8
DO - 10.1016/S0378-4347(98)00083-8
M3 - Article
SN - 1572-6495
VL - 709
SP - 310
EP - 314
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
IS - 2
ER -