Determination of cocaine and its metabolites in human urine by gas chromatography/mass spectrometry after simultaneous use of cocaine and ethanol

Rafael de la Torre, Jordi Ortuño, M. Luisa González, Magí Farré, Jordi Camí, Jordi Segura

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20 Citations (Scopus)

Abstract

Cocaethylene is an active metabolite produced when cocaine is consumed jointly with ethanol. The development of analytical techniques for determining cocaethylene and other cocaine metabolites is highly relevant for pharmacokinetic and toxicology studies of the cocaine and alcohol interaction in humans. The gas chromagraphy/mass spectrometry (GC/MS) method here reported is based on a single solid-phase extraction together with deuterated internal standards previously added to urine, followed by derivatization with pentafluoropropionic anhydride (hydroxyl and amine functions) and 1,1,1,3,3,3 hexafluor-2-propanol (carboxylic acid function) and injection into a capillary GC system coupled to a mass spectrometric detector in the selected ion monitoring acquisition mode. A sensitivity of 1-2 ng ml-1 for the quantitative analysis of cocaine and its main metabolites (ecgonine methyl ester, benzoylecgonine, cocaethylene and norcocaine) was achieved. In addition, some other minor metabolites were easily extracted and detected. © 1995.
Original languageEnglish
Pages (from-to)305-312
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Jan 1995

Keywords

  • capillary gas chromatography/mass spectrometry
  • cocaethylene
  • Cocaine and alcohol interaction
  • norcocaethylene
  • urinary metabolic profile

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