Determination of ammonium and L-Glutamine in hybridoma cell cultures by sequential flow injection analysis

Carles Campmajó, Jordi Joan Cairó, Anna Sanfeliu, Esteve Martínez, Salvador Alegret, Francesc Gòdia

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24 Citations (Scopus)

Abstract

A flow injection anlytical system based on a gas diffusion membrane module for ammonia and an ammonium flow-through potentiometric detector has been set up for measurement of L-glutamine and ammonium ions in hybridoma cell cultures. The main feature of the system is that the same basic analytical concept and equipment is used in both measurements, the only difference being for the determination of L-glutamine, in which the sample flows through an immobilized glutaminase cartridge. The conditions to enable the performance of both analysis consecutively, avoiding potential interferences by unwanted deamination of other compounds in the samples, have been determined. Finally, the proposed system has been compared with reference analytical methods for batch hybridoma cell culture experiments. © 1994 Kluwer Academic Publishers.
Original languageEnglish
Pages (from-to)177-182
JournalCytotechnology
Volume14
Issue number3
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • ammonium
  • flow injection analysis
  • hybridoma culture
  • L-glutamine
  • sequential determination

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