Determination of β-HCG using amperometric immunosensors based on a conducting immunocomposite

M. Santandreu, S. Alegret, E. Fàbregas

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An amperometric immunosensor based on a rigid immunocomposite was built to measure human chorionic gonadotropin β-subunit (β-HCG). The immunocomposite is formed by a conducting graphite-methacrylate matrix containing the anti-β-HCG antibody. The main advantage of this kind of immunosensors is their easy regeneration which can be achieved by a simple polishing of their surface. β-HCG is determined with a sandwich assay using anti-β-HCG conjugate labelled with horseradish peroxidase (HRP). The extent of the immunological interaction is quantified by the activity of the labelling enzyme: peroxidase is regenerated after the enzymatic reduction of H2O2 by hydroquinone which is used as a mediator in the solution. The reduction of hydroquinone is amperometrically monitored using a potential of -0.1V. Each assay can be realised in less than 40min. The upper limit corresponds to a β-HCG concentration of 98.0IU/l and the detection limit to 2.6IU/l. Copyright (C) 1999 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)181-188
JournalAnalytica Chimica Acta
Issue number2-3
Publication statusPublished - 20 Sep 1999


  • β-HCG determination
  • Graphite-methacrylate immunocomposite
  • Human chorionic gonadotropin
  • Immunosensor
  • Renewable immunosensor


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