Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome

Maria Calsamiglia; Joaquim Segalés; Josefina Quintana; Carles Rosell; Mariano Domingo

doi:https://doi.org/10.1128/JCM.40.5.1848-1850.2002

Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome

Maria Calsamiglia, Joaquim Segalés, Josefina Quintana, Carles Rosell, Mariano Domingo

Department of Animal Health and Anatomy

Research output: Contribution to journal › Article › Research › peer-review

65 Citations (Scopus)

Abstract

Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)- affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.

Original language	English
Pages (from-to)	1848-1850
Journal	Journal of Clinical Microbiology
Volume	40
DOIs	https://doi.org/10.1128/JCM.40.5.1848-1850.2002
Publication status	Published - 20 May 2002

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title = "Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome",

abstract = "Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)- affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.",

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Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. / Calsamiglia, Maria; Segalés, Joaquim; Quintana, Josefina; Rosell, Carles; Domingo, Mariano.

In: Journal of Clinical Microbiology, Vol. 40, 20.05.2002, p. 1848-1850.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome

AU - Calsamiglia, Maria

AU - Segalés, Joaquim

AU - Quintana, Josefina

AU - Rosell, Carles

AU - Domingo, Mariano

PY - 2002/5/20

Y1 - 2002/5/20

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AB - Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)- affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.

U2 - https://doi.org/10.1128/JCM.40.5.1848-1850.2002

DO - https://doi.org/10.1128/JCM.40.5.1848-1850.2002

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