Detection of induced anti-sperm antibodies by an improved enzyme-linked immunosorbent assay

J. M. Benet-Rubinat, P. Martinez, W. A. Lepp, J. Egozcue, P. Andolz, M. A. Bielsa

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12 Citations (Scopus)


The enzyme-linked immunosorbent assay (ELISA) has been adapted for the detection of human anti-sperm antibodies (ASA). The use of peroxidase in the enzyme-anti-immunoglobulin conjugate, together with a nontoxic chromogenic substrate (MBTH-DMAB), renders the assay highly sensitive for detection of sperm surface antigens. The ELISA is highly reproducible, not only intra-assay (94% to 97%) but also inter-assay (80% to 96%). A great advantage of this ELISA is that microplates can be kept at -20°C for months without any decrease in the response. New Zealand white rabbits showed a significant response to human sperm and tail fractions obtained by sonication. The sperm were extremely immunogenic, reaching titers of antisera of 1/10,000 or more. The results show that this ELISA is a good tool for the objective measurement of the anti-sperm response, and is significantly more sensitive than other techniques for anti-sperm antibody analysis.
Original languageEnglish
Pages (from-to)48-56
JournalInternational Journal of Fertility
Issue number1
Publication statusPublished - 1 Jan 1991


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