Detection of impaired DNA replication and repair in micronuclei as indicators of genomic instability and chromothripsis

Mariona Terradas, Marta Martín, Anna Genescà

Research output: Chapter in BookChapterResearchpeer-review

1 Citation (Scopus)

Abstract

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The physical isolation of chromosomes within micronuclei offers an attractive mechanistic explanation for the local DNA fragmentation and clustered genome rearrangements that characterize chromothripsis. Localized shattering of the chromatin confined in micronuclei can be a consequence of defects in micronuclei basic general functions, such as DNA replication and repair. The detection of DNA repair and replication defects in micronuclei is described here, as well as the analysis of chromosome breakage and inaccurate reassembly of broken segments in the daughter cells, as indirect methods to detect chromothripsis.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages197-208
Number of pages11
Volume1769
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Chromosome rearrangements
  • Chromothripsis
  • DNA fragmentation
  • Micronuclei

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