Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: Implications for the detection of minimal residual disease in patients with small cell lung cancer

Montserrat Torà; Víctor M. Barberá; F. X. Real

doi:https://doi.org/10.1016/S0304-3835(00)00577-2

Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: Implications for the detection of minimal residual disease in patients with small cell lung cancer

Montserrat Torà, Víctor M. Barberá, F. X. Real

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/106 non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n = 6) and patients with SCLC (n = 5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	157-164
Journal	Cancer Letters
Volume	161
DOIs	https://doi.org/10.1016/S0304-3835(00)00577-2
Publication status	Published - 20 Dec 2000

Keywords

HuD
Minimal residual disease
Neuroblastoma
Reverse transcriptase-polymerase chain reaction
Small cell lung cancer

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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@article{7ae9bbcf38ba4c67801eb8a2a7d2c986,

title = "Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: Implications for the detection of minimal residual disease in patients with small cell lung cancer",

abstract = "Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/106 non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n = 6) and patients with SCLC (n = 5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "HuD, Minimal residual disease, Neuroblastoma, Reverse transcriptase-polymerase chain reaction, Small cell lung cancer",

author = "Montserrat Tor{\`a} and Barber{\'a}, {V{\'i}ctor M.} and Real, {F. X.}",

year = "2000",

month = dec,

day = "20",

doi = "https://doi.org/10.1016/S0304-3835(00)00577-2",

language = "English",

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pages = "157--164",

journal = "Cancer Letters",

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}

Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: Implications for the detection of minimal residual disease in patients with small cell lung cancer. / Torà, Montserrat; Barberá, Víctor M.; Real, F. X.

In: Cancer Letters, Vol. 161, 20.12.2000, p. 157-164.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: Implications for the detection of minimal residual disease in patients with small cell lung cancer

AU - Torà, Montserrat

AU - Barberá, Víctor M.

AU - Real, F. X.

PY - 2000/12/20

Y1 - 2000/12/20

N2 - Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/106 non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n = 6) and patients with SCLC (n = 5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. (C) 2000 Elsevier Science Ireland Ltd.

AB - Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/106 non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n = 6) and patients with SCLC (n = 5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. (C) 2000 Elsevier Science Ireland Ltd.

KW - HuD

KW - Minimal residual disease

KW - Neuroblastoma

KW - Reverse transcriptase-polymerase chain reaction

KW - Small cell lung cancer

U2 - https://doi.org/10.1016/S0304-3835(00)00577-2

DO - https://doi.org/10.1016/S0304-3835(00)00577-2

M3 - Article

VL - 161

SP - 157

EP - 164

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

ER -