Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/106 non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n = 6) and patients with SCLC (n = 5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. (C) 2000 Elsevier Science Ireland Ltd.
- Minimal residual disease
- Reverse transcriptase-polymerase chain reaction
- Small cell lung cancer