Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction

Mónica Escarceller; Francisco Rodriguez-Frias; Rosendo Jardi; Blanca San Segundo; Ramon Eritja

doi:10.1016/S0003-2697(05)80007-7

Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction

Mónica Escarceller, Francisco Rodriguez-Frias, Rosendo Jardi, Blanca San Segundo, Ramon Eritja

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.

Original language	English
Pages (from-to)	36-42
Journal	Analytical Biochemistry
Volume	206
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2697(05)80007-7
Publication status	Published - 1 Jan 1992

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title = "Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction",

abstract = "Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. {\textcopyright} 1992 Academic Press, Inc.",

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Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction. / Escarceller, Mónica; Rodriguez-Frias, Francisco; Jardi, Rosendo; San Segundo, Blanca; Eritja, Ramon.

In: Analytical Biochemistry, Vol. 206, No. 1, 01.01.1992, p. 36-42.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Eritja, Ramon

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AB - Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.

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