Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction

Mónica Escarceller, Francisco Rodriguez-Frias, Rosendo Jardi, Blanca San Segundo, Ramon Eritja

    Research output: Contribution to journalArticleResearchpeer-review

    19 Citations (Scopus)

    Abstract

    Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.
    Original languageEnglish
    Pages (from-to)36-42
    JournalAnalytical Biochemistry
    Volume206
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 1992

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