TY - JOUR
T1 - Detection of hepatitis B virus DNA in human serumsamples: Use of digoxigenin-labeled oligonucleotides as modified primers for the polymerase chain reaction
AU - Escarceller, Mónica
AU - Rodriguez-Frias, Francisco
AU - Jardi, Rosendo
AU - San Segundo, Blanca
AU - Eritja, Ramon
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.
AB - Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.
U2 - 10.1016/S0003-2697(05)80007-7
DO - 10.1016/S0003-2697(05)80007-7
M3 - Article
VL - 206
SP - 36
EP - 42
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -
