TY - JOUR
T1 - Detection of charge-perturbation of tryptophan residues by fourth derivative spectrophotometry
AU - Sabés, Manuel
AU - Padrós, Esteve
PY - 1987/1/1
Y1 - 1987/1/1
N2 - Abstract— Fourth‐derivative spectrophotometry has been applied to the analysis of the 1La O‐O band of the indole chromophore. Using several tryptophan models, it is shown that when this band is the most red‐shifted, it produces a minimum at about 300 nm in the fourth‐derivative spectrum. In proteins this red shift and the 300‐nm minimum results from a charge‐perturbation effect acting through space on the indole group of tryptophan residues. It is concluded that fourth‐derivative spectrophotometry can be used for monitoring the effect of charges on tryptophan residues Copyright © 1987, Wiley Blackwell. All rights reserved
AB - Abstract— Fourth‐derivative spectrophotometry has been applied to the analysis of the 1La O‐O band of the indole chromophore. Using several tryptophan models, it is shown that when this band is the most red‐shifted, it produces a minimum at about 300 nm in the fourth‐derivative spectrum. In proteins this red shift and the 300‐nm minimum results from a charge‐perturbation effect acting through space on the indole group of tryptophan residues. It is concluded that fourth‐derivative spectrophotometry can be used for monitoring the effect of charges on tryptophan residues Copyright © 1987, Wiley Blackwell. All rights reserved
U2 - 10.1111/j.1751-1097.1987.tb07394.x
DO - 10.1111/j.1751-1097.1987.tb07394.x
M3 - Article
SN - 0031-8655
VL - 45
SP - 657
EP - 661
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 5
ER -