Detection of charge-perturbation of tryptophan residues by fourth derivative spectrophotometry

Manuel Sabés, Esteve Padrós

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)


Abstract— Fourth‐derivative spectrophotometry has been applied to the analysis of the 1La O‐O band of the indole chromophore. Using several tryptophan models, it is shown that when this band is the most red‐shifted, it produces a minimum at about 300 nm in the fourth‐derivative spectrum. In proteins this red shift and the 300‐nm minimum results from a charge‐perturbation effect acting through space on the indole group of tryptophan residues. It is concluded that fourth‐derivative spectrophotometry can be used for monitoring the effect of charges on tryptophan residues Copyright © 1987, Wiley Blackwell. All rights reserved
Original languageEnglish
Pages (from-to)657-661
JournalPhotochemistry and Photobiology
Issue number5
Publication statusPublished - 1 Jan 1987


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