Detection of biomarkers with carbon nanotube-based immunosensors.

Samuel Sánchez; Esteve Fàbregas; Martin Pumera

doi:10.1007/978-1-60761-579-8_19

Detection of biomarkers with carbon nanotube-based immunosensors.

Samuel Sánchez, Esteve Fàbregas, Martin Pumera

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

Original language	English
Pages (from-to)	227-237
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	625
DOIs	https://doi.org/10.1007/978-1-60761-579-8_19
Publication status	Published - 1 Jan 2010

Access to Document

10.1007/978-1-60761-579-8_19

Cite this

@article{6be0b22bf1cf46f6a5d9a1700ee12ec8,

title = "Detection of biomarkers with carbon nanotube-based immunosensors.",

abstract = "A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.",

author = "Samuel S{\'a}nchez and Esteve F{\`a}bregas and Martin Pumera",

year = "2010",

month = jan,

day = "1",

doi = "10.1007/978-1-60761-579-8_19",

language = "English",

volume = "625",

pages = "227--237",

}

TY - JOUR

T1 - Detection of biomarkers with carbon nanotube-based immunosensors.

AU - Sánchez, Samuel

AU - Fàbregas, Esteve

AU - Pumera, Martin

PY - 2010/1/1

Y1 - 2010/1/1

N2 - A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

AB - A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

U2 - 10.1007/978-1-60761-579-8_19

DO - 10.1007/978-1-60761-579-8_19

M3 - Article

VL - 625

SP - 227

EP - 237

ER -

Detection of biomarkers with carbon nanotube-based immunosensors.

Abstract

Access to Document

Fingerprint

Cite this