Detection of anti-sperm antibodies in serum, seminal plasma and cervical mucus by the immunobead test

P. Andolz, M. A. Bielsa, P. Martínez, V. García-framis, J. M. Benet-rubinat, J. Egozcue

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Anti-sperm antibodies in serum and seminal plasma were detected by means of an indirect immunobead test (IBT). Immunobeads with separate specificities for each immuno-globulin class (IBT-IgG, IBT-IgM, and IBT-IgA) were used. Semen parameters were controlled in all sperm donors and Biggers-Whitten-Whittingham (BWW) medium supplemented with human serum albumin (HSA) was used to increase sperm motility. This technique was tested with high titre anti-human sperm sera induced in rabbits. Sperm tails showed a good response by IBT. We included in this study 178 men and 35 women evaluated for infertility and the sera were also tested by the Tray Agglutination Test (TAT). Although the presence of semen markers such as agglutination or trembling of spermatozoa is meaningful even by itself, the percentage of anti-sperm antibodies was increased in the patients with markers, both using IBT (21.4%) and using TAT (35.7%). At high titres of specific immunoglobulins (rabbit antisera and vasectomized men), the correlation between IBT and TAT techniques was better than in sera with very low titres, in which more positive TAT's were detected. © 1990 Oxford University Press.
Original languageEnglish
Pages (from-to)685-689
JournalHuman Reproduction
Issue number6
Publication statusPublished - 1 Jan 1990


  • Anti-sperm antibodies
  • Cervical mucus
  • Immunobead test
  • Seminal plasma
  • Serum


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