Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity

Mercè Badia; Enrique Querol

doi:10.1016/0166-0934(88)90084-5

Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity

Mercè Badia, Enrique Querol

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. © 1988.

Original language	English
Pages (from-to)	23-29
Journal	Journal of Virological Methods
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(88)90084-5
Publication status	Published - 1 Jan 1988

Keywords

Bovine herpesvirus 1
Glycoprotein
Lectin

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10.1016/0166-0934(88)90084-5

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title = "Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity",

abstract = "A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. {\textcopyright} 1988.",

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AU - Badia, Mercè

AU - Querol, Enrique

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N2 - A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. © 1988.

AB - A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. © 1988.

KW - Bovine herpesvirus 1

KW - Glycoprotein

KW - Lectin

U2 - 10.1016/0166-0934(88)90084-5

DO - 10.1016/0166-0934(88)90084-5

M3 - Article

VL - 22

SP - 23

EP - 29

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1

ER -

Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity

Abstract

Keywords

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