© Springer Science+Business Media, LLC, part of Springer Nature 2019. Yeasts are efficient cell factories and are commonly used for the production of recombinant proteins for biopharmaceutical and industrial purposes. For such products high levels of correctly folded proteins are needed, which sometimes requires improvement and engineering of the expression system. The article summarizes major breakthroughs that led to the efficient use of yeasts as production platforms and reviews bottlenecks occurring during protein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||20|
|Publication status||Published - 1 Jan 2019|
|Name||Methods in Molecular Biology|
- Protein degradation
- Protein production
Zahrl, R. J., Gasser, B., Mattanovich, D., & Ferrer, P. (2019). Detection and elimination of cellular bottlenecks in protein-producing yeasts. In Methods in Molecular Biology (Vol. 1923, pp. 75-95). (Methods in Molecular Biology; Vol. 1923). https://doi.org/10.1007/978-1-4939-9024-5_2