Detection and elimination of cellular bottlenecks in protein-producing yeasts

Richard J. Zahrl, Brigitte Gasser, Diethard Mattanovich, Pau Ferrer

Research output: Chapter in BookChapterResearchpeer-review

5 Citations (Scopus)

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2019. Yeasts are efficient cell factories and are commonly used for the production of recombinant proteins for biopharmaceutical and industrial purposes. For such products high levels of correctly folded proteins are needed, which sometimes requires improvement and engineering of the expression system. The article summarizes major breakthroughs that led to the efficient use of yeasts as production platforms and reviews bottlenecks occurring during protein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages75-95
Number of pages20
Volume1923
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1923

Keywords

  • Chaperones
  • Metabolism
  • Promoters
  • Protein degradation
  • Protein production
  • Secretion
  • Yeasts

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    Zahrl, R. J., Gasser, B., Mattanovich, D., & Ferrer, P. (2019). Detection and elimination of cellular bottlenecks in protein-producing yeasts. In Methods in Molecular Biology (Vol. 1923, pp. 75-95). (Methods in Molecular Biology; Vol. 1923). https://doi.org/10.1007/978-1-4939-9024-5_2