Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México

Marco Antonio Cantú-Martínez; Artur Xavier Roig-Sagués; Sibilina Cedillo-Rosales; Diana Elisa Zamora-Ávila; Ramiro Ávalos-Ramírez

doi:10.1590/S0036-36342013000200011

Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México

Marco Antonio Cantú-Martínez, Artur Xavier Roig-Sagués, Sibilina Cedillo-Rosales, Diana Elisa Zamora-Ávila, Ramiro Ávalos-Ramírez

Department of Animal and Food Science

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.

Original language	English
Pages (from-to)	193-195
Journal	Salud Publica de Mexico
Volume	55
Issue number	2
DOIs	https://doi.org/10.1590/S0036-36342013000200011
Publication status	Published - 23 May 2013

Keywords

Hepatitis E
HEV
Mexico
Pork livers

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

Access to Document

10.1590/S0036-36342013000200011

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@article{6c2e156842684b21a20f3e4b6cd668cb,

title = "Detecci{\'o}n molecular del virus de la hepatitis E en h{\'i}gados de cerdo destinados al consumo humano en el estado de Nuevo Le{\'o}n, M{\'e}xico",

abstract = "Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.",

keywords = "Hepatitis E, HEV, Mexico, Pork livers",

author = "Cant{\'u}-Mart{\'i}nez, {Marco Antonio} and Roig-Sagu{\'e}s, {Artur Xavier} and Sibilina Cedillo-Rosales and Zamora-{\'A}vila, {Diana Elisa} and Ramiro {\'A}valos-Ram{\'i}rez",

year = "2013",

month = may,

day = "23",

doi = "10.1590/S0036-36342013000200011",

language = "English",

volume = "55",

pages = "193--195",

number = "2",

}

Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México. / Cantú-Martínez, Marco Antonio; Roig-Sagués, Artur Xavier; Cedillo-Rosales, Sibilina; Zamora-Ávila, Diana Elisa; Ávalos-Ramírez, Ramiro.

In: Salud Publica de Mexico, Vol. 55, No. 2, 23.05.2013, p. 193-195.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México

AU - Cantú-Martínez, Marco Antonio

AU - Roig-Sagués, Artur Xavier

AU - Cedillo-Rosales, Sibilina

AU - Zamora-Ávila, Diana Elisa

AU - Ávalos-Ramírez, Ramiro

PY - 2013/5/23

Y1 - 2013/5/23

N2 - Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.

AB - Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.

KW - Hepatitis E

KW - HEV

KW - Mexico

KW - Pork livers

U2 - 10.1590/S0036-36342013000200011

DO - 10.1590/S0036-36342013000200011

M3 - Article

VL - 55

SP - 193

EP - 195

IS - 2

ER -