TY - JOUR
T1 - Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México
AU - Cantú-Martínez, Marco Antonio
AU - Roig-Sagués, Artur Xavier
AU - Cedillo-Rosales, Sibilina
AU - Zamora-Ávila, Diana Elisa
AU - Ávalos-Ramírez, Ramiro
PY - 2013/5/23
Y1 - 2013/5/23
N2 - Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.
AB - Objective. Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. Materials and methods. 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. Results. 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. Conclusions. Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.
KW - Hepatitis E
KW - HEV
KW - Mexico
KW - Pork livers
U2 - 10.1590/S0036-36342013000200011
DO - 10.1590/S0036-36342013000200011
M3 - Article
VL - 55
SP - 193
EP - 195
IS - 2
ER -