Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Cinzia Spinato, Aritz Perez Ruiz De Garibay, Magdalena Kierkowicz, Elzbieta Pach, Markus Martincic, Rebecca Klippstein, Maxime Bourgognon, Julie Tzu Wen Wang, Cécilia Ménard-Moyon, Khuloud T. Al-Jamal, Belén Ballesteros, Gerard Tobias, Alberto Bianco

    Research output: Contribution to journalArticleResearchpeer-review

    15 Citations (Scopus)

    Abstract

    © The Royal Society of Chemistry 2016. In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
    Original languageEnglish
    Pages (from-to)12626-12638
    JournalNanoscale
    Volume8
    Issue number25
    DOIs
    Publication statusPublished - 7 Jul 2016

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